福建农业学报2011，Vol.26Issue(2)：143-147,5.
水稻T-DNA插入启动子捕获系的初步筛选
Preliminary Screening of Rice Promoter Trap Lines with T-DNA Tagging
摘要
Abstract
Binary vector pCAMBIA1300GUSA-Hyg containing β-glucuronidase (GUS) without the promoter was introduced into Oryza sativa L. Minghui 86 by agrobacterium-mediated transformation. The χ2 tests on the hygromycin resistant ratio revealed that 28 of the 39 rice promoter trap lines contained a loci T-DNA tagging. GUS histochemical assay at different stages in different organs, such as root, stem, leave, anther, hull, embryo and emdospern, of 2 653 rice promoter trap lines was performed. The results showed that 83 rice promoter lines were GUS positive. Of all tested, 24 lines were expressed in endosperm (i. e. , 0. 91 ％) and 19 lines expressed in anther (i. e. , 0. 72％). Information collected on the rice promoter trap lines could be useful in identifying new rice genes.关键词
水稻/启动子捕获系/T-DNA标签Key words
Rice/ promoter trap lines/ T-DNA tagging分类
生物科学引用本文复制引用
胡昌泉,刘华清,李素一,潘大仁,王锋..水稻T-DNA插入启动子捕获系的初步筛选[J].福建农业学报,2011,26(2):143-147,5.基金项目
福建省科技计划--省属公益类科研院所基本科研专项(2009R10035-3) （2009R10035-3）
福建省自然科学基金项目(2010J01099) （2010J01099）
