国际病理科学与临床杂志2011，Vol.31Issue(3)：185-192,8.DOI:10.3969/j.issn.1673-2588.2011.03.001
激光捕获显微切割纯化的人正常支气管上皮与肺鳞癌组织的定量蛋白质组学研究
Quantitative proteomic study for purified human lung squamous carcinoma and normal human bronchial epithelial by laser capture microdissection
摘要
Abstract
Objective To establish the differential protein profile for human lung squamous carcinoma (HLSC) and normal human bronchial epithelium (NHBE). Methods Laser capture microdissection (LCM) was used to purify the target cells from HLSC tissues and matched NHBE. A stable isotope labeled strategy using iTRAQ together with 2 dimensional chromatography/hybrid quadrupole time of flight (2D-LC/Q-STAR) mass spectrometry was performed to separate and identify the differential expression proteins. Results A total of 96 differential expression proteins in the LCM-purified HLSC and NHBE were identified. Compared with NHBE, 49 proteins were upregulated while 47 proteins were downregulated in HLSC. The expression levels of the differential proteins HSPB1 and CKB were confirmed by Western blot analysis, which were consistent with the results of quantitative proteomics. Conclusion The different expression proteins in HLSC will provide useful information for exploring tle carcinogenic mechanism of HLSC.关键词
肺鳞癌/差异蛋白质/激光捕获显微切割/定量蛋白质组学/iTRAQKey words
lung squamous carcinoma/ differential protein/ laser capture microdissection/quantitative proteomics/ iTRAQ引用本文复制引用
许艳,曾谷清,金龙玉,汤参娥,李萃,肖志强..激光捕获显微切割纯化的人正常支气管上皮与肺鳞癌组织的定量蛋白质组学研究[J].国际病理科学与临床杂志,2011,31(3):185-192,8.基金项目
国家自然科学基金(30972970) （30972970）
教育部新世纪优秀人才培养计划基金(2007-0861) （2007-0861）
湖南省自然科学基金(08JJ6010) （08JJ6010）
湖南省卫生厅科研基金(B2007-068 （）
B2010-037) （）
湖南省教育厅科研基金(09C837). （09C837）
