解剖学杂志2011,Vol.34Issue(3):294-297,4.
大鼠大网膜Ⅰ型和Ⅱ型乳斑的确定及对比
Identification and comparison between omental type Ⅰ and type Ⅱ milky spots of the greater omentum in rat
摘要
Abstract
Objective: To identify the type of milky spots, and investigate the similarities and differences between type Ⅰ and type Ⅱ milky spots in number, area and cell composition. Methods: Double-labeling of rat omental milky spots with activated carbon and H-E staining were used to measure the number and the area of type Ⅰ and type Ⅱ milky spots by Image-Pro plus software, and the differences in ultrastructure between type Ⅰ and type Ⅱ milky sports were observed under an electron microscope. Results: In physiological conditions, the number of the type Ⅱ milky spots was more significant than that of the type Ⅰ milky spots [(9. 153±0. 818)per cm2 vs (1. 913±0. 851)per cm2 , P<0. 01], and the area of the type Ⅱ milky spots was significantly larger than that of the type Ⅰ milky spots [(0. 421±0.136)mm2 vs (0. 102±0.041)mm2 , P <0. 01]. Two types of milk spots were mainly composed of macrophages, lymphocytes, mast cells, monocytes, plasma cells, eosinophils, reticular cells and stromal cells; vessel, lymph vessel and adipocyte were observed in type Ⅱ milky spots.Activated carbon particles were observed in macrophages of two types milky spots. Conclusion: Both activated carbon and H-E staining can mark milky spots; Omental milky spots can be classified into two types: type Ⅰ and type Ⅱ (type Ⅰ milky spots are located in the thin serous membrane of the omentum and no blood vessels or adipose tissues are found in the surrounding area; type Ⅱ milky spots are distributed close to vascular networks and adipose tissues, curved vessel can be observed in this milky spots). Two types of the milky spots in the tissue cell composition are different due to the distribution; Macrophages in both types of milky spots have the immune function of phagocytizing foreign particles.关键词
大网膜乳斑/分型/电镜/大鼠Key words
omental milky spots/ classification/ electron microscopy/ rat引用本文复制引用
李尚胜,黄允宁..大鼠大网膜Ⅰ型和Ⅱ型乳斑的确定及对比[J].解剖学杂志,2011,34(3):294-297,4.基金项目
宁夏自然科学基金重点项目(NZ10160) (NZ10160)