解剖学杂志2011，Vol.34Issue(3)：354-357,387,5.
二烯丙基二硫与硼替佐米联合应用诱导成神经细胞瘤细胞凋亡及活性氧的生成
Apoptosis and ROS production induced by diallyl disulfide in combination with bortezomib in neuroblastoma cells
摘要
Abstract
Objective: To explore the apoptosis of neuroblastoma cells and the change of ROS induced by diallyl disulfide (DADS) in combination with bortezomib.Methods: Neuroblastoma SH-SY5Y cells were treated with DADS, bortezomib and DADS plus bortezomib.The cell viability was detected by methyl thiazolyl tetrazolium (MTT), and Hoechst-33528 stainning was employed to observe the apoptosis morphology.The production of ROS and the apoptotic rate of cells were measured by a flow cytometry method.Results: The combination of DADS and bortezomib increased the apoptosis of neuroblastoma cells,and their combination produced more ROS than when either was used alone.Conclusion: Bortezomib can facilitate the apoptosis of neurobtastoma induced by DADS; The enhanced production of ROS plays an important role during this process.关键词
活性氧/硼替佐米/二烯丙基二硫/成神经细胞瘤/细胞凋亡Key words
reactive oxygen species/ bortezomib/ diallyt disulfide/ neuroblastoma/cell apoptosis引用本文复制引用
刘蛟,李京敏,李振中,黄飞..二烯丙基二硫与硼替佐米联合应用诱导成神经细胞瘤细胞凋亡及活性氧的生成[J].解剖学杂志,2011,34(3):354-357,387,5.基金项目
山东省自然科学基金(2009ZRB01288) （2009ZRB01288）
滨州医学院科研计划与科研启动基金(BY2009KJ10) （BY2009KJ10）
