广西植物2011，Vol.31Issue(3)：377-382,421,7.DOI:10.3969/j.issn.1000-3142.2011.05.018
铬(Cr6+)对水花生愈伤组织的毒害效应
Toxic of chromium(Cr )on the callus of Alternanthera philoxeroides
摘要
Abstract
This study focused on changes of several representative physiological characteristics and ultrastructure in the callus of Alternanthera philoxreroides under different concentrations of Cr6+ stress(0,0. 1,0. 2,0. 5,1.5 mmol ·L -1 ). The results showed that with the increasing of Cr6 +, (1) total chlorophyll (Chl), chlorophyll a/b ( Chl a/b) and total soluble protein contents showed the same trend,which increased firstly and then decreased. (2)Superoxide anion ( O2- · ) and hydrogen peroxide ( H2 O2 ) content irritably increased compared to the control group, then decreased, however, it's always higher than that of control. (3)The soluble sugar content enhanced with the increasing of Cr6 + concentration. (4) Activities of supeoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) and ASA and GSH contents all increased first and then decreased. (5)Transmission electron microscope observation indicated that Cr6+ also imposed injury action on ultrastructure of callus cells., thylakoid twisted, chloroplast enyelope broke and large starch grain formed,mitochondria disappeared and formed vacuolization, nucleolus lost and chromatin condensed in nucleus,and endoplasmic reticulum vanished by means of vesiculation. The conclusion could be reached that toxic effects of Cr6+ on callus resulted in disorder of physiological and biochemical metabolism and destruction of ultrastructure of callus cells.关键词
Cr6+/水花生/愈伤组织/生理生化Key words
Cr6+/ Alternanthera philoxeroides/ callus/ characteristics分类
农业科技引用本文复制引用
张乐乐,施国新,王娟,康宜宁,徐小颖,陈辉..铬(Cr6+)对水花生愈伤组织的毒害效应[J].广西植物,2011,31(3):377-382,421,7.基金项目
国家自然科学基金(30870139) （30870139）
