广西植物2011，Vol.31Issue(3)：403-406,4.DOI:10.3969/j.issn.1000-3142.2011.05.023
梨蒴珠藓愈伤组织诱导和配子体再生研究
Studies on callus induction and gametophyte regeneration of Bartramia pomi formis
付素静 1方炎明 2张启香2
作者信息
- 1. 贵州省铜仁学院,生化系,贵州,铜仁,554300
- 2. 南京林业大学,森林资源与环境学院,南京,210037
- 折叠
摘要
Abstract
This research was conducted to select a suitable culture system for the callus induction and gametophyte regeneration of Bartrarnia pomiformis. And axenic mosses were inoculated on MS and Knop solid media with different hormone combilations for callus and buds induction and differentiation. The result showed that MS medium containing 0. 5 mg/L BA,0. 1 mg/L 2,4-D was optimal for callus induction,with the induction rate of 33.3％. Optimal medium for inducing gametophyte is MS+ 1.0 mg/L BA,and the achieved multiplication coefficient was 10.0. And Knop with no hormone was as well as MS in generation of gametophyte. This investigation could provide some important references for callus induction and systematics of B. pomiformis.关键词
梨朔珠藓/组织培养/愈伤组织/配子体再生Key words
Bartramia pomiforrnis/ in vitro culture/ callus/ gametophyte regeneration分类
农业科技引用本文复制引用
付素静,方炎明,张启香..梨蒴珠藓愈伤组织诱导和配子体再生研究[J].广西植物,2011,31(3):403-406,4.
