热带作物学报2011，Vol.32Issue(3)：437-442,6.DOI:10.3969/j.issn.1000-2561.2011.03.014
荔枝果皮2个POD同源基因的生物信息学分析及酶活测定
Bioinformatics Analysis and Function Forecast of Two POD Genes in Litchi chinensis Pericarp
摘要
Abstract
Two POD genes were cloned from the pericarp of Litchi chinensis and named as LiPOD1 and LiPOD2,respectively.Analysis indicated that the full length of LiPOD1 was 1 062 bp with an open reading frame encoding 353 amino acids that shared high identities with the POD from Hevea brasiliensis, Populus trichocarpa, lpomoea batatas(87％, 84％ and 81％.The full length of LiPOD2 was 990 bp with an open reading frame encoding 329 amino acids that shared high identities with the POD from Gossypium hirsutum, Sesamum indicum, Medicago sativa, Arabidopsis tha//ana(88％, 87％, 85％ and 81％).Analysis of conserved domains indicated that LiPOD1 and LiPOD2 both had total conserved domains of POD gene and had heine binding site, active site, substrate binding site and calcium binding site.During the process of skin browning within 72 hours both the browning index and POD activity increased.Analysis of structure of LiPOD1 and LiPOD2 speculated that these two genes played important roles in the process of pericarp senescence and browning in pestharvest Litchi chinensis.关键词
荔枝果皮/过氧化物酶/序列分析/褐变指数/POD活性Key words
Litchi chinensis pericarp/ peroxidase/ sequence analysis/ browning index/ POD activity分类
农业科技引用本文复制引用
郑雯,张永丽,王家保,金志强..荔枝果皮2个POD同源基因的生物信息学分析及酶活测定[J].热带作物学报,2011,32(3):437-442,6.基金项目
现代农业技术体系建设专项资金资助(nycytx-32-06). （nycytx-32-06）
