热带作物学报2011，Vol.32Issue(3)：447-451,5.DOI:10.3969/j.issn.1000-2561.2011.03.016
番茄XRN2基因的克隆、表达分析及遗传转化
Clonging, Expression Analysis and Genetic Transformation of XRN2 Gene in Tomato
摘要
Abstract
To understand the function of XRN2 gene in tomato, the full length SIXRN2 cDNA was amplified by RT-PCR from tomato mix cDNAs. Expression analysis results showed that SlXRN2 expressed the highest in tomato roots. The expression of XRN2 gene could be up-regulated by wound stress on the leaves. Then SlXRN2 gene driven by the constitutive promoter CaMV35S was directed cloning into plant expression vector pCambia1301-35S,named as pCambia1301-35S-XRN2 and pCambia1301-35S-AsXRN2. Transgenic tomato lines were obtained via agrobacterium-mediated transformation, which would provide significant evidence for further study of XRN2 function in tomato关键词
XRN2/半定量PCR/伤害处理/载体构建/遗传转化/番茄Key words
XRN2/ Semi-quantitative PCR/ Wound treatment/ Construction of expression vector/, Genetic transformation/Tomato分类
农业科技引用本文复制引用
刘俊江,李正国,杨迎伍,先志强,邓伟..番茄XRN2基因的克隆、表达分析及遗传转化[J].热带作物学报,2011,32(3):447-451,5.基金项目
国家自然科学基金(No.30972002,31071798)和中央高校基本科研业务费(No.CDJXS102300). （No.30972002,31071798）
