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红掌组培苗遗传变异的RAPD检测技术研究

潘英文 林明光 刘福秀

热带作物学报2011,Vol.32Issue(3):468-470,3.
热带作物学报2011,Vol.32Issue(3):468-470,3.DOI:10.3969/j.issn.1000-2561.2011.03.020

红掌组培苗遗传变异的RAPD检测技术研究

Detection of genetic Variation of Anthurium andraeanum Planlet bv RAPD

潘英文 1林明光 1刘福秀1

作者信息

  • 1. 海南出入境检验检疫局热带植物隔离检疫中心,海南海口570311
  • 折叠

摘要

Abstract

The research was to detect the genetic variation in A nthurium andraeanum. One hundred tissue culturing seedlings of Pink Champion variety sub - cultured 6 times were selected randomly for RAPD analyses. Seven of 50 random primers were used in this study for its stabilized amplification. Forty- eight clear bands were amplified from the samples mentioned above and 1 sample varied in the bands. So the genetic variation rate of the samples was 1%. The research results would provide rapid, economical and accurate detection of genetic variation for industrial seedling in A nthurium andraeanum.

关键词

红掌/组培苗/变异检测/RAPD

Key words

A nthurium andraeanum./ Tissue culturing seedlings/ Variation detection/ RAPD

分类

农业科技

引用本文复制引用

潘英文,林明光,刘福秀..红掌组培苗遗传变异的RAPD检测技术研究[J].热带作物学报,2011,32(3):468-470,3.

基金项目

国家科技支撑计划项目(No.2007BAD45B07),海南省重大研发专项(No.080201 (No.2007BAD45B07)

2009YFZX003). ()

热带作物学报

OA北大核心CSCDCSTPCD

1000-2561

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