烟草科技Issue(5)：74-79,6.
TMV、PVY福建分离物分子鉴定及末端区域变异分析
Molecular Identification and Variation Analysis of Terminal Region for TMV and PVY Isolates from Fujian
摘要
Abstract
To accurately and timely identify viral pathogens and track variation and epidemic trend of viral diseases, specific primers were used in RT-PCR, by which TMV and PVY were identified respectively from infected tobacco leaves collected from Fujian Province.The results of viral sequence analysis showed that the amplification products were the 5' terminal region of TMV and the 3' terminal region of PVY with sequence lengths of 959 and 646 nt respectively, both including of terminal noncoding region and part of the coding region of viral functional genetic protein.Comparing the obtained terminal sequence with the same regions of the viral isolates in NCBI revealed that the noncoding region of 5 ' terminal end of TMV was highly conservative, N terminal coding region of some of 126kDa or 183kDa protein among different TMV isolates presented some variations (mutations).In terms of numerical differences of variation, the difference between CP coding region and 3 ' terminal region of PVY was not apparent.The variations (mutations) mainly came from base conversion.Natural selection pressures affected virus genome variation (mutation) to a certain degree.关键词
烟草普通花叶病毒(TMV)/马铃薯Y病毒(PVY)/RT-PCR/分子鉴定/变异Key words
Tobacco mosaic virus (TMV) / Potato virus Y(PVY) / RT-PCR/ Molecular identification/ Variation分类
农业科技引用本文复制引用
杨军,张俊祺,罗朝鹏,赖荣泉,曾文龙,李锋,宋纪真,尹启生..TMV、PVY福建分离物分子鉴定及末端区域变异分析[J].烟草科技,2011,(5):74-79,6.基金项目
国家烟草专卖局科技项目、郑州烟草研究院科研项目"烟草主要病毒病分子变异监控及追踪技术研究(122009CZ0410)". （122009CZ0410）
