生态环境学报2011，Vol.20Issue(4)：716-721,6.
酱油曲霉(Aspergillus sojae)利用甘蔗渣产絮凝剂条件筛选及优化
Screening and optimizing the conditions of bioflocculant production from Aspergillus sojae in sugarcane bagasse culture
摘要
Abstract
The significant factors influencing the growth, quality and quantity of bioflocculant have been screened out through Placket-Burmah experiment to investigate the nutritional and culture conditions for the production of bioflocculant by Aspergillus sojae using sugarcane bagasse as carbon source. The screened significant factors were then optimized by monofactorial experiment.The PB experimental results showed that the concentration of K2HPO4, reducing sugar and the culture time were significant for the growth of the strain, and the initial pH of culture was notable factor for flocculant activity while the concentrations of reducing sugar and (NH4)2SO4 influenced the crude yield significantly. The single-factor experiments demonstrated that the optimum nutritional and culture conditions were enzymatic hydrolysate of sugarcane bagasse with (13.67±0.54) g·L-1 reducing sugar, 4 g·L-1 (NH4)2SO4,initial pH 5, temperature 30 ℃ and culture time 60 h.关键词
Plackett-Burman实验/甘蔗渣/酱油曲霉/微生物絮凝剂Key words
Plackett-Burman experiment/ Sugarcane bagasse/ Aspergillus sojae/ bioflocculant分类
资源环境引用本文复制引用
冯绮澜,尹华,秦华明,叶锦韶,彭辉,张娜,何宝燕,彭素芬..酱油曲霉(Aspergillus sojae)利用甘蔗渣产絮凝剂条件筛选及优化[J].生态环境学报,2011,20(4):716-721,6.基金项目
国家水体污染控制与治理重大科技专项(2008ZX07211-003) （2008ZX07211-003）
广东省科技计划项目(2008B030302038) （2008B030302038）
番禺区科技局科技计划项目(2009-Z-64-1) （2009-Z-64-1）
