中国水稻科学2011，Vol.25Issue(3)：249-255,7.DOI:10.3969/i.issn.1001-7216.2011.03.004
双胚苗水稻单倍体及其杂交后代基因组DNA甲基化特异位点的分析及功能探讨
Analysis of Special DNA Methylated Sites Between Haploid of Twin-Seedling and Its Hybrids in Rice
摘要
Abstract
The DNA cytosine methylation mutation at the 5'-CpCpGpG sites of the haploid of SARII-628, and its hybrids with Shuhui 527, Shuhui 363 were analyzed by employing the MSAP (methylation sensitive amplification polyphisim) method. A total of 765 DNA methylated sites were detected and the methylation level of hybrids was lower than that of the parents.Meanwhile, different bands between hybrids and parents were analyzed and two types of methylated sites were detected, of which one inherited from the haploid, and the other didn't. The biological functions of genes related to methylated sites involved in cell structure, metabolize and stress reaction. Therefore, DNA methylated modifications may be involved in the regulation of related genes, playing an important role in plant growth, development and envolution.关键词
双胚苗水稻/单倍体/甲基化敏感性扩增多态性/DNA甲基化Key words
twin-seedling rice/ haploid/ methylation sensitive amplification polymorphism/ DNA methylation分类
农业科技引用本文复制引用
吴绍华,薛晶晶,张红宇,徐培洲,吴先军..双胚苗水稻单倍体及其杂交后代基因组DNA甲基化特异位点的分析及功能探讨[J].中国水稻科学,2011,25(3):249-255,7.基金项目
国家自然科学基金资助项目(30771157,30971618) （30771157,30971618）
四川省科技厅项目(2008JY0094). （2008JY0094）
