中国水稻科学2011，Vol.25Issue(3)：331-334,4.DOI:10.3969/j.issn.1001-7216.2011.03.016
通过分子标记辅助选择技术选育携有水稻白叶枯病抗性基因Xa23的水稻株系
Breeding of Rice Lines with Bacterial Blight Resistance Gene Xa23 by Using Marker-Assisted Selection
摘要
Abstract
The rice bacterial blight resistance gene Xa23, which is carried by the donor parent 18113, was introduced into the intersubspecific restorer line H705 and good grain quality restorer line H706 by crossing and molecular marke-assisted selection(MAS). Seventy-one and 52 homozygous lines carrying Xa23 were obtained from the 18113/H705 F3 and 18113/H706 F3 lines, respectively by PCR with EST marker C189. The bacterial blight resistance of these lines was investigated with the pathotype Ⅳ representative strain Zhe 17.3 by clipping method. Sixty-one and 44 lines showed the bacterial blight resistance,with the MAS accuracies of 85.92％ and 84.61％, respectively. The C189 was one of the effective MAS markers for testing Xa23 gene. In addition, further research is needed to elucidate the mechanisms of higher false positive rate than theoretical exchange rate.关键词
水稻/白叶枯病/分子标记辅助选择/抗性基因/育种Key words
rice/ bacterial blight/ marker-assisted selection/ resistance gene/ breeding分类
农业科技引用本文复制引用
范宏环,王林友,张礼霞,于新,王曦,金庆生,王建军..通过分子标记辅助选择技术选育携有水稻白叶枯病抗性基因Xa23的水稻株系[J].中国水稻科学,2011,25(3):331-334,4.基金项目
国家自然科学基金重点项目(2007AA022162) （2007AA022162）
国家863计划资助项目(2010AA101302) （2010AA101302）
国家科技支撑计划资助项目(2011BAD35B02) （2011BAD35B02）
浙江省0406汁划资助项目(2007C12901-2). （2007C12901-2）
