实用临床医药杂志2011，Vol.15Issue(13)：5-7,11,4.
p38MAPK在大鼠局灶性脑缺血再灌注损伤中的作用
The role of p38 MAPK in focal cerebral ischemia/reperfusion injury in rats
王军 1左其龙 1徐汉荣1
作者信息
- 1. 江苏省盐城市第一人民医院,神经内科,江苏,盐城,224000
- 折叠
摘要
Abstract
Objective To study the mechanism of p38 MAPK after focal cerebral ischemia/reperfusion injury in rats. Methods Healthy male Sprague -Dawley rats were randomly divided into five groups: normal control group, sham group, ischemia/reperfusion group, SB202190 group and DMSO group. Except normal control group and sham group, rats' cerebral ischemia/reperfusion model was established with suture method. In SB202190 group and DMSO group, SB202190 (the specific inhibitor of p38 MAPK) and 1％ of dimetyl sulphoxide (DMSO) were injected into the lateral ventricle respectively. The expression of Bcl - 2 and Bax protein was studied and the behavioral score was recorded. Results ① Behavioral results: rats' nerve function score of control group and sham group decreased after ischemia - reperfusion 24 h. Neurological function in SB202190 group was higher than in IR group, and there was statistical significance(P ＜0.01 ). ② Western blot test results: both Bcl-2 and Bax protein all express a few in normal control group and sham group, but there was no difference ( P ＞ 0.05 ). The expression of Bcl - 2 protein decreased in IR group but there was no obvious statistical difference, Bax protein increased obviously at 24 h after ischemia/reperfusion (P ＜ 0.05 ). Compared with IR group, the expression of bax protein in SB202190 group decreased, and there was obvious increases of Bcl - 2 protein in SB202190 group ( P ＜ 0.05 ). Conclusion Inhibition of the p38 MAPK can protect neural function from being injured after focal cerebral ischemia/reperfusion injury in rats.关键词
p38 MAPK/缺血再灌注损伤/SB202190/Bcl-2/BaxKey words
p38 MAPK/ ischemia/reperfusion injury/ SB202190/ Bcl-2/ Bax分类
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王军,左其龙,徐汉荣..p38MAPK在大鼠局灶性脑缺血再灌注损伤中的作用[J].实用临床医药杂志,2011,15(13):5-7,11,4.
