激光生物学报2011，Vol.20Issue(3)：292-298,7.DOI:10.3969/j.issn.1007-7146.2011.03.003
氦氖激光和亚硝基胍复合诱变选育高产乙醇的休哈塔假丝酵母
Selection of High Ethanol Producing Candida shehatae by Combined Mutagenesis with He-Ne Laser and NTG
摘要
Abstract
Ethanol fermentation of xylose has been known as the key factor for lignocellulose to produce ethanol. Candida shehatae was one of the better natural yeast with the capability to ferment xylose. Combined mutagenesis with He-Ne laser and NTG were studied on Candida shehatae HDYXHT-Ol in order to select strains of high ethanol by ferment xylose. After the mutagenesis with He-Ne laser, the HN-3 was obtained with ethanol production and yield rate were 17.03 g/L and 0. 3393 g/g respectively, which was increased by 20.36 percent compared with the original strain. The HN-3 was continuous mutagenesis by NTG, an mutant strain NTG-2 with ethanol production of 24.20 g/L was selected,which was increased by 42.10 percent compared with HN-3. The NTG-2 was incubated with shaking it; flask for fermentation for 48 h, the ethanol production, xylose utilization, ethanol yield rate and ethanol production rate reached 24.16 g/L, 69.26 ％, 0.4360 g/g and 0.7075 g/(Loh), respectively.关键词
休哈塔假丝酵母/氦氖激光诱变/NTG诱变/乙醇Key words
Candida shehatae/ He-Ne laser mutation/ NTG mutation/ ethanol分类
生物科学引用本文复制引用
葛菁萍,孙红兵,王轶男,宋刚,平文祥..氦氖激光和亚硝基胍复合诱变选育高产乙醇的休哈塔假丝酵母[J].激光生物学报,2011,20(3):292-298,7.基金项目
国家自然科学基金(31070446) （31070446）
国家高技术研究发展计划项目(2007AA100702-6) （2007AA100702-6）
黑龙江省教育厅重点项目(11551zO11) （11551zO11）
黑龙江省科技攻关重大项目(GA07B401-6) （GA07B401-6）
哈尔滨市科技创新人才研究专项资金项目(RC2010XK002028) （RC2010XK002028）
黑龙江大学高层次人才(创新团队)支持计划(Hdtd2010-17)资助 （创新团队）
