激光生物学报2011，Vol.20Issue(3)：309-314,6.DOI:10.3969/j.issn.1007-7146.2011.03.006
ALA-PDT对正常人角质形成细胞生物效应的实验研究
An Experimental Research on ALA-PDT Inhibit Normal Human Keratinocytes Cell Proliferation
摘要
Abstract
Objective: To explore the feasibility as well as the optimum clinical effect of ALA-PDT inhibiting the proliferation of kerationocytes cell (KC).Methods: Dispase isolated enzymes and trypsin digestion two-step method were used to prepare and cultivate KC from normal human foreskin after circumcision.The experiment was divided into control group, ALA only group, light only group, and 0.1 mmol/L, 0.6 mmoL/L, 1.2 mmol/L, 1.8 mmol/L, 3.6 mmol/L five concentrations of ALA-PDT groups having 0.5 h、1 h、3 h、5 h four periods of incubation time.We used the enzyme mark instrument to examine KC survival and fluorescence microscope was used to examine the fluorescence intensity of protoporphyrin Ⅸ of KC.Then the effect of ALA-PDT on KC apoptosis and its growth cycle were observed by using AO staining fluorescence examination and Annexin Ⅴ / PI double staining methods.Results:0.6 mmol/L of ALA-PDT group was the optimal concentration and 1 h was the optimum incubation time, showing the highest fluorescence intensity and highest rate of apoptosis.It also had the greatest impact on the Phase S and Phase G2 of KC' s growth cycle, which led to the lowest cell proliferation index.Conclusion:0.6 mmol/L ALA-PDT with 1 h incubation time can significantly inhibit the proliferation of KC, and effectively promote normal human KC apoptosis.关键词
ALA-PDT/角质形成细胞/凋亡Key words
ALA-PDT/ keratinocytes/ apoptosis分类
医药卫生引用本文复制引用
谢光辉,李康英,刘宏伟,李嘉佩..ALA-PDT对正常人角质形成细胞生物效应的实验研究[J].激光生物学报,2011,20(3):309-314,6.基金项目
广东省医学科研基金立项课题(A2008361) （A2008361）
