激光生物学报2011，Vol.20Issue(3)：338-341,4.DOI:10.3969/j.issn.1007-7146.2011.03.012
枸杞多糖对H5N1流感全病毒灭活疫苗的体液免疫增强作用
Antibody Responses Induced by Inactivated Whole-virion H5N1 Vaccine with Lycium barbarum Polysaccharide as an Adjuvant
摘要
Abstract
Humoral immune effects induced by inactivated whole-virion H5N1 vaccine adjuvanted with Lycium barbarum polysaccharide (LBP) were explored in BALB/c mice.Mice were intraperitoneally immunized with the whole-virion inactivated influenza virus A/Vietnam/1194/2004 ( H5N1 ) vaccine formulated with different dosages of Lycium barbarum polysaccharide (LBP).Three weeks after the immunization, mice were bled for detection of serum vaccine-specific antibody levels.Mice injected with aluminium hydroxide-adjuvanted inactivated vaccine were used as positive control.Results showed that serum specific antibody titers in mice were increased along with the increasing amount of LBP within a certain range.Compared with the non-adjuvanted vaccine, the vaccine adjuvanted with LBP at dosage of 800 μg significantly enhanced the antibody level.The adjuvant effect of LBP on humoral responses was comparable to that of aluminium hydroxide.LBP may be a potential adjuvant to inactivated influenza virus vaccines.关键词
枸杞多糖/流感/灭活疫苗/佐剂Key words
Lycium barbarum polysaccharide/ influenza/ inactivated vaccine/ adjuvant分类
医药卫生引用本文复制引用
刘为,许馨月,张风华,陈则,方芳..枸杞多糖对H5N1流感全病毒灭活疫苗的体液免疫增强作用[J].激光生物学报,2011,20(3):338-341,4.基金项目
国家支撑计划(2006BAD06A03) （2006BAD06A03）
湖南省科技厅科研计划项目(2006NK2003) （2006NK2003）
湖南省教育厅一般项目(07C564) （07C564）
上海市科委科研计划项目(064319030) （064319030）
