激光生物学报2011，Vol.20Issue(3)：353-359,7.DOI:10.3969/j.issn.1007-7146.2011.03.015
砀山酥梨石细胞发育过程中PPO酶学特性及其cDNA片段克隆
PPO Characterization and a PPO cDNA Coloning during the Development of Stone Cell in Pyrus bretschneideri cv.
摘要
Abstract
The enzymatic characteristics of Polyphenol oxidase (PPO) affected the formation of stone cell were investigated and the PPO gene was cloned with the material of fruit of Pyrus bretschneideri cv.The results showed that during the development of Pyrus bretschneideri tv., PPO activity reached peaks in 27th and 47th days after anthesis (DAA) respectively, which appeared earlier than the peaks of contents of lignin ( in 47 DAA and 61 DAA) and stone cell ( in 51 DAA)respectively, meanwhile, the variation of PPO activites had extremely significant correlation for both of them.The optimum conditions of PPO activity were established, namely, pH value 7.0, temperature 40 ℃ and substrate concentration 0.16 mol/L.The kinetics of enzymatic browning reaction in this experiment was conformed to Michaelis equation and the kinetic parameters Km and Vmax were 0.1364 mol/L and 454.5455 U/g respectively.The fragment of 558 bp cDNA of the PPO gene was cloned from the fruit organ of Pyrus bretschneideri cv.and its sequence was submitted to the GenBank database with accession No.HQ329065.Sequence analysis and homology comparison revealed that the fragment of PPO gene from Pyrus bretschneideri cv.had a high correlation with other plant species.关键词
砀山酥梨/多酚氧化酶/木质素/石细胞/酶学特性/RT-PCRKey words
Pyrus bretschneideri cv./ PPO/ lignin/ stone cell/ enzyme characteristics/ RT-PCR分类
生物科学引用本文复制引用
李玲,于娟娟,金青,蔡永萍,林毅,吕芮宏..砀山酥梨石细胞发育过程中PPO酶学特性及其cDNA片段克隆[J].激光生物学报,2011,20(3):353-359,7.基金项目
国家自然科学基金(30771483) （30771483）
