激光生物学报2011，Vol.20Issue(3)：409-412,387,5.DOI:10.3969/j.issn.1007-7146.2011.03.025
地衣芽孢杆菌D-1产碱性蛋白酶培养条件的优化
Optimization of Culture Conditions of Alkaline Protease Production by Bacillus licheniformis D-1
摘要
Abstract
In order to optimize culture condition for alkaline protease production by Bacillus licheniformis D-1, the culture temperature, pH, stirring speed and air flow rate during fermentation were optimized by the L9 (34) orthogonal test in 10 L bioreactor. The optimized culture conditions were determined as a culture temperature of 37℃, a pH of 7.5, an air flow rate 4 L/min, and a stirring speed of 300 r/min. Under these optimization conditions, the confirmative experiment shows that the activity of alkaline protease produced by Bacillus licheniformis D-1 reached 91.26 U/mL.关键词
地衣芽孢杆菌/碱性蛋白酶/优化Key words
Bacillus licheniformis/ alkaline protease/ optimization分类
生物科学引用本文复制引用
刘海进,钱坤,李吕木,许发芝,丁小玲,景志远..地衣芽孢杆菌D-1产碱性蛋白酶培养条件的优化[J].激光生物学报,2011,20(3):409-412,387,5.基金项目
国家科技部科技人员服务企业行动项目(2009GJC30021) （2009GJC30021）
安徽省农业科技成果转化项目(09150306006) （09150306006）
