陕西医学杂志2011，Vol.40Issue(7)：771-774,4.
抗c-kit特异性siRNA对肺泡巨噬细胞介导的炎性因子生成的影响
Effect of anti-c-kit gene specific siRNA on inflammatory cytokines mediated by alveolar macrophages
摘要
Abstract
Objective: To explore the effects of suppression on the c-Kit gene expression by RNA interference on the production of inflammatory cytokines mediated by alveolar macrophage activated by mite allergen.Methods: Alveolar macrophages obtained through bronchoalveolar lavage.CD4+ T cells were separated from peripheral blood with methods of Ficoll-Hypaque density-gradients centrifugation and magnetic cell sorting system (MACS).CD4+ T cells were co-cultured with alveolar macrophages activated by the mite allergen.Using specific siRNA inhibited the expression of c-kit gene.Flow Cytometry and Weatern blot detected effects of gene silence and Enzyme-Linked Immunosorbent Assay measured the production of inflammatory cytokines in culture supernatants.Results: siRNA could be transferred into the alveolar macrophages effectively with the aid of transfection agent and significantly inhibited the expression of c-Kit protein, and decreased the production of inflammatory cytokines.Conclusion: Anti-c-kit-siRNA can reduce the production of inflammatory cytokines mediated by alveolar macrophages.关键词
巨噬细胞,肺泡/免疫学/原癌基因蛋白质c-kit/免疫学/炎症介导素类/免疫学/RNA干扰/哮喘Key words
Macrophages, Alveolar/immunology/ Proto-Oncogene/ Protein c-kit/immunology/ Inflammation mediators/immunology/ @RNA interence/ Asthma分类
医药卫生引用本文复制引用
兀威,刘凯歌,刁鑫,陈晖,刘延峰,曹博淦..抗c-kit特异性siRNA对肺泡巨噬细胞介导的炎性因子生成的影响[J].陕西医学杂志,2011,40(7):771-774,4.基金项目
陕西省科技厅自然科学基金项目(2009JM4033) （2009JM4033）
