陕西医学杂志2011，Vol.40Issue(7)：781-784,4.
原发性干燥综合征患者唇腺组织与外周血BAFF/BAFF-R检测及临床意义
Expression of BAFF/BAFF-R in labial salivary glands/peripheral blood from pSS patients and its significance
杨西超 1吴振彪 1朱平 1王彦宏1
作者信息
- 1. 第四军医大学西京医院临床免疫科,西安,710032
- 折叠
摘要
Abstract
Objective : To identify the important role of BAFF (B-cell activating factor from TNF family,BAFF) in the pathogenesis of pSS (Primary Sjogren's syndrome, pSS) and further to explore the diagnostic value of BAFF-R (BAFF Receptor,BAFF-R) for SS by detecting the expression of BAFF in serum and BAFF-R on peripheral B cells/labial salivary glands from SS patients.methods :The expression of BAFF-R was detected on labial salivary glands and in CD19+ B cells in peripheral blood from 35 pSS patients by immunohistochemistry and flow cytometry, respectively.The concentration of soluble BAFF in serum was detected by ELISA.The labial salivary glands and peripheral blood from 35 healthy donors served as controls.Results: BAFF-R waa strongly stained on labial salivary glands from 32/35 pSS-paients ( 91.4％), while weakly stained on that in the controls.BAFF-R was also expressed significantly higher in CD19+ B cells from patients (24.6 ± 8.3 ％ vs 3.8 ± 2.2 ％).Similar to these, soluble BAFF was highly detected in sera of patients than that in the controls (8.2±1: 1.1 ng/ml vs 1.9 ± 0.7 ng/ml).Conclusion: Our study further identified that up-regulation of BAFF and BAFF-R may play an important role in the pathogenesis of pSS by activating self-reactive B cells , and we also provide some primary evidence for the diagnosis of pSS by detecting BAFF-R on labial salivary glands.关键词
干燥综合征/免疫学/B淋巴细胞活化因子/淋巴细胞活化因子受体/唇/B淋巴细胞Key words
Sjogren's syndrome/immunology/ @BAFF/ @BAFF-R/ Lip/ B-Iymphocytes分类
医药卫生引用本文复制引用
杨西超,吴振彪,朱平,王彦宏..原发性干燥综合征患者唇腺组织与外周血BAFF/BAFF-R检测及临床意义[J].陕西医学杂志,2011,40(7):781-784,4.
