实用临床医药杂志2011，Vol.15Issue(11)：1-4,4.
阿托伐他汀钙预防大鼠肝缺血再灌注损伤的实验研究
The effects of Atorvastatin on liver ischemia-reperfusion injury in rats
翁爱彬 1赵剑锋 1王宇军 1卓莹 1崔国通1
作者信息
- 1. 莆田学院附属医院,药剂科,福建,莆田,351100
- 折叠
摘要
Abstract
Objective To explore the protective effect of atorvastatin on liver and its possible mechanism during the liver ischemia - reperfusion injury. Methods Single liver in site ischemia - reperfusion rats model was used. Twenty- one healthy male SD rats were randomly divided into three groups, each consisting of 7 rats: sham operate group (N), ischemia-reperfusion group (I/R) and atorvastation treatment group (AT + I/R). After 90 minutes' reperfusion, blood was collected in each group by the superior and inferior vena cava of liver so as to detect alanine aminotransferase (ALT) and asparate aminotransferase (AST). Liver homogenate was centrifuged at low temperature, and then the content variation of superoxide dismustase (SOD), malondialdehyde (MDA), myeloPeroxidase (MPO), nitric oxide (NO) and nitrieoxidesynthas (NOS) in liver tissue were measured by kit. Meanwhile, liver tissue was observed by light microscope. Results Compared group AT + I/R with group I/R: ALT [ (928.43 ± 96.17) vs. (1 234.89 ± 327.17) ] IU/L;AST [(1 222.91±249.85) vs (1 635.74±481.86)] IU/L; MDA [(2.35±0.57) vs. (3.29± 1.23)] nmol/mg; MPO [(2.33±0.71) vs. (3.18±0.82)] IU/mg; NO [(89.27±8.27) vs.(102.32±11.81)] μmol/mg; iINOS [(0.25±0.14)vs. (0.45±0.21)] U/mg decreased (P＜0.05) and SOD [(20.86±2.49) vs (14.58±2.04)] IU/mg; eNOS [(0.69±0.17) vs. (0.56±0.13)] U/mg increased (P＜0.05). Conclusion Atorvastatin could protect liver from ischemia-reperfusion injury. Its possible mechanisms are activating antioxidant free radicals and improving the effects of organisms, that is, scavenging oxygen free radicals and anti - inflammatory.关键词
阿托伐他汀钙/缺血再灌注损伤/大鼠/肝脏Key words
Atorvastatin/ ischemia- reperfusion/ rats/ liver分类
医药卫生引用本文复制引用
翁爱彬,赵剑锋,王宇军,卓莹,崔国通..阿托伐他汀钙预防大鼠肝缺血再灌注损伤的实验研究[J].实用临床医药杂志,2011,15(11):1-4,4.
