实用临床医药杂志2011，Vol.15Issue(11)：5-7,14,4.
异甘草酸镁对大鼠肝缺血-再灌注损伤后肝细胞凋亡及bcl-2表达的影响
Effect of magnesium isoglycyrrhizinate on liver apoptosis and bcl-2 expression during hepatic ischemia reperfusion injury in rats
裴旭东 1翟玉峰 1张怀宏 1叶启发2
作者信息
- 1. 河南省南阳市中心医院,传染科,河南,南阳,473000
- 2. 武汉大学中南医院,普外科,湖北,武汉,430071
- 折叠
摘要
Abstract
Objective To explore the effect of magnesium isoglycyrrhizinAte (MI) on liver apoptosis and expression of bcl -2 during hepatic ischemia reperfusion injury(HIRI) in rats. Methods Forty SD rats were randomly divided into I/R group ( n = 20) and MI + I/R group ( n = 20). We detected the concentration of MDA and SOD at 1,3, 6, 24 h point after HIRI, and detected the liver apoptosis and the expression level of bcl -2 1 h after HIRI by TUNEL and Immunohistochemistry respectively. Results The concentration of MDA gradually increased in I/R group while gradually decreased in MI + I/R group(P ＜0.05 or P ＜0.01 ). The concentration of SOD increased gradually in both groups, and at 3 h and 4 h point, SOD in MI + I/R group was significantly higher than that in I/R group(P ＜0.01 ). The number of positive nucleus and the percentage of positive liver nucleus in I/R + MI group were significantly less than those in I/R group respeetively(P ＜0.01 ). The number of bcl - 2 protein positive liver cells and the percentage of bcl - 2 protein positive liver cells in MI + I/R group were significantly more than those of I/R group respectively (P ＜ 0.01 ). Conclusion MI decreases liver apoptosis after HIRI by inhibiting the production of oxygenic free radicals and up - regulating the expression level of bcl -2. So MI has promising therapeutic effect on HIRI.关键词
缺血再灌注/肝细胞凋亡/bcl-2基因/异甘草酸镁Key words
ischemic repeffusion/ liver apoptosis/ bcl - 2 gene/ magnesium isoglycyrrhizinate分类
医药卫生引用本文复制引用
裴旭东,翟玉峰,张怀宏,叶启发..异甘草酸镁对大鼠肝缺血-再灌注损伤后肝细胞凋亡及bcl-2表达的影响[J].实用临床医药杂志,2011,15(11):5-7,14,4.
