中国中西医结合杂志2011，Vol.31Issue(7)：932-936,5.
中药双黄补对人牙周膜细胞在玉米醇溶蛋白支架材料上黏附生长及增殖的影响
Effect of Shuanghuangbu on the Adhesion Growth and Proliferation of Human Periodontal Ligament Cells on the Zein Stent
摘要
Abstract
Objective To study the effect of Shuanghuangbu on the adhesion growth and proliferation of human periodontal ligament cells (HPDLCs) on the zein scaffold, and to study the feasibility of Shuanghuangbu zein used in the periodontal tissue engineering as a compound scaffold material. Methods HPDLCs were cultured in vitro using the explants adherent method. Shuanghuangbu Decoction was prepared using water extraction and alcohol precipitation method. Zein scaffold was prepared using solvent casting/corpuscle leaching method, which was dispensed into Shuanghuangbu culture fluid at the concentration of 50,100, 150,200,500, 1 000 μg/mL, respectively. Shuanghuangbu of different concentrations groups, the zein scaffold leaching liquid group, and the cell control group were also set up. The effect of each group on the proliferation of HPDLCs was detected by MTT. According to results of MTT, the best concentration of Shuanghuangbu (100 μg/mL) was selected as the experiment group. Ten percent FBS of DMEM culture fluid was taken as the control group. The effect of Shuanghuangbu on the morphology of HPDLCs on the zein scaffold was observed using inverted phase contrast microscope and scanning electron microscope. Results Compared with the zein scaffold leaching liquid group and the cell control group,the proliferation of HPDLCs could be promoted by different concentrations of Shuanghuangbu culture fluid, with the most obvious effect shown by Shuanghuangbu at 100 μg/mL (P ＜0. 01 ). There was no obvious difference between the zein scaffold leaching liquid group and the cell control group (P＞0. 05), but their cell proliferation rates were positive values. The morphological results demonstrated that HPDLCs successfully grew on the zein scaffold. The number of HPDLCs under the action of Shuanghuangbu was more and the morphous was better. Conclusions Shuanghuangbu could promote the adhesion growth and proliferation of HPDLCs on the zein scaffold. Shuanghuangbu zein was feasible to be used in the periodontal tissue engineering as a compound scaffold materia.关键词
人牙周膜细胞/双黄补/玉米醇溶蛋白/支架材料/细胞增殖Key words
human periodontal ligament cell/ Shuanghuangbu/ zein/ scaffold/ the cell proliferation引用本文复制引用
许彦枝,李娜,刘健,吴景景,杨凤英,罗冬青..中药双黄补对人牙周膜细胞在玉米醇溶蛋白支架材料上黏附生长及增殖的影响[J].中国中西医结合杂志,2011,31(7):932-936,5.基金项目
国家自然科学基金资助项目(No.30873289) （No.30873289）
