国际医药卫生导报2011，Vol.17Issue(15)：1793-1796,4.DOI:10.3760/cma.j.issn.1007-1245.2011.15.001
结核杆菌复苏促进因子在分枝杆菌培养中的作用观察
The role of TB-resuscitation promoting factor in culture of mycobacterium tuberculosis
摘要
Abstract
Objective To explore the role of TB-resuscitation promoting factor proteins ( TB-RPFs )in culture of mycobacterium tuberculosis. Methods 42 sputum samples, 8 samples of bronchoalveolar lavage fluid ( BALF ) and 25 samples of pleural fluid for tuberculosis patients were collected .All samples were treated with 4%NaOH, washed with PBS and then suspended. Each sample was divided into control group and RPF group. Control group was cultured in MGIT, RPF group was cultured with five types of TB-RPFs in MGIT. All samples were cultured in the automated mycobacteria testing M960 system.The cultivation time for positive cultures was recorded and the positive cultures were used to identify mycobacterium strains. Results The positive rate of sputum culture was 42.86% in the RPF group and 45.24% in the control group; that of BALF was 37.5% and 62.5%, respectively; and the positive rate of pleural fluid was 20% in both groups. There were no significant differences between the two groups. Of all the positive samples, 9 had the same days for positive results.6 samples ( 3 pleural fluid, 2 sputum, and 1 BALF ) were reported positive earlier in the RPF group than the control group, with an average length of 4.33 days but,9 were reported positive later in the RPF group group with an average length of 1.11 days. 3 samples were cultured positive in the control group but negative in the RPF group.Conclusions There is no enough evidence to prove that TB-RPFs can improve the positive rate for mycobacterium culture in sputum, BALF and pleural fluid samples. But TB-RPFs significantly shorten the positive culture time in pleural fluid. The role of TB-RPFs for culturing mycobacterium in different samples needs to be further studied.关键词
结核杆菌/复苏因子/分离培养Key words
Mycobacterium tuberculosis/Resuscitation promoting factor/Isolated culture引用本文复制引用
郑小凌,蔡杏珊,谢贝,孟繁荣,高俊文,尹小毛,周辉林,刘志辉..结核杆菌复苏促进因子在分枝杆菌培养中的作用观察[J].国际医药卫生导报,2011,17(15):1793-1796,4.基金项目
广东省医学科研基金资助立项(B2009215),广州市医药卫生科技一般引导项目(2008-YB-095) （B2009215）
