国际医药卫生导报2011，Vol.17Issue(16)：1933-1936,4.DOI:10.3760/cma.j.issn.1007-1245.2011.16.002
人博卡病毒广州分离株全基因组序列测定及分析
Detection and analysis on complete genomic sequence of human bocavirus GZ2010-1 strain in Guangzhou
摘要
Abstract
Objective To study the structural features and different types of the genomic molecular organization of human bocavirus in infected infants in Guangzhou. Methods Primers were designed on the basis of the genomic sequence of human bocavirus BJ3064 strain (DQ988933) in the GenBank, and amplify HBOV genome by PCR.The PCR-products were directly sequenced. The genomic nucleotide sequences were analyzed with the programs of CLUSTAL W/X and MEGA5.0.Results The strain GZ2010-1 genome is 5299bp in length. The genomic sequences of GZ2010-1 strain were compared with those sequences of HBOV1, HBOV2, HBOV3, and HBOV4 genotypes in GenBank, revealed that the homology with HBOV1 genotype ranged from 98% to 99%, homology with genotypes HBOV2, HBOV3, HBOV4 were 76%, 81% ,and 75% respectively. The F genes of GZ2010-1 strain was compared with F genes of HBOV1 genotype, showed that the homology was also between 98%-99%. Conclusions The complete nucleotide sequence of human bocavirus GZ2010-1 strain isolated from Guangzhou is 5299 bp in length,GenBank accession NO.is JN 128956. Sequence and phylogenetic analysis of the GZ2010-1 strain reveales that this isolate belongs to HBOV1.关键词
人博卡病毒/基因组/序列分析/广州Key words
Human bocavirus/Genome/Sequence analysis/Guangzhou引用本文复制引用
钟家禹,朱冰,施静,邓力,陈翊,谢嘉慧,张莹莹..人博卡病毒广州分离株全基因组序列测定及分析[J].国际医药卫生导报,2011,17(16):1933-1936,4.基金项目
广州市卫生局立项课题(2009-YB-215) （2009-YB-215）
