大豆科学2011，Vol.30Issue(3)：369-373,5.
大豆未成熟种子子叶节不定芽再生的初步研究
Shoots Regeneration of Cotyledon Node from Soybean Immature Seeds
摘要
Abstract
Immature seed cotyledons nodes of Jidou 12 and Zhonghuang 13 were used as explants to research the effect of 6-BA concentration, time of obtaining materials and degree of seeds development on the proportion of shoots regeneration from immature seeds cotyledons nodes. The result showed that the best time of obtaining materials of Jidou 12 was 60 - 66 d after flowering, the optimal concentration of 6-BA in multiple shoots induction medium was 1.0 - 1.5 mg · L -1 ,and the proportion of shoots reached 96.67％ - 100％. The best time of obtaining materials of Zhonghuang 13 was 49-65 d after flowering, the optimal concentration of 6-BA in multiple shoots induction medium was 1.5 mg · L-1 ,and the proportion of shoots reached 93.33％ - 100％. Proportion of shoots by immature seeds cotyledons nodes were higher than mature seeds cotyledons nodes by 4％ and 10％ for Jidou 12 and Zhonghuang 13, respectively.关键词
大豆/未成熟种子子叶节/诱导Key words
Soybean/ Immature seeds cotyledon node/ Induction分类
农业科技引用本文复制引用
王卢平,乔亚科,李桂兰,钟磊,崔姗姗,纪展波..大豆未成熟种子子叶节不定芽再生的初步研究[J].大豆科学,2011,30(3):369-373,5.基金项目
转基因生物新品种培育重大专项资助项目(2009ZX08004-001B （）
2009ZX08004-004B) （）
河北省自然科学基金资助项目(C2009000868) （C2009000868）
河北省科技支撑计划资助项目(09220103D). （09220103D）
