大豆科学2011，Vol.30Issue(3)：374-378,5.
不同基因型大豆Fd-GOGAT基因cDNA序列的克隆与分析
Cloning and Analysis of Fd-GOGAT Genes from Different Soybean Varieties
摘要
Abstract
Fd-GOGAT genes from six different soybean varieties were cloned. The comparative analysis showed the sequences of GOGAT genes in different soybean varieties were very identical, so we can design general real-time PCR primer to detect express regulation of Fd-GOGAT genes. The coding sequence of Fd-GOGAT gene in Dongnong42 appeared an absence of 8 nucleotides, which brought frameshift mutation,led to its C-terminal lack of 79 amimo acids conserved sequence and the GS1 big subunit C-terminal conserved domain( gltB_C )were not in their integrity. Molecular and protein evolution tree analysis showed Dongnong 42, Dongnong 46 and G. gracilis had similar evolution distance for Fd-GOGAT gene.关键词
大豆/Fd-GOGAT基因/克隆Key words
Soybean/ Fd-GOGAT genes/ Enrichment separation/ Clone分类
农业科技引用本文复制引用
陈丽华,刘丽君,刘页丽,祖伟..不同基因型大豆Fd-GOGAT基因cDNA序列的克隆与分析[J].大豆科学,2011,30(3):374-378,5.基金项目
国家"十一五"科技攻关资助项目(2006BAD21B01). （2006BAD21B01）
