大豆科学2011，Vol.30Issue(3)：442-446,5.
根际促生菌诱导大豆抗大豆胞囊线虫的生化机理
Biochemical Mechanism of Resistance against Soybean Cyst Nematode Induced by Plant Growth Promoting Rhizobacteria in Soybean
摘要
Abstract
It has been a new research focus on biological control that the induction of disease resistance and growth response in plants is elicited by plant growth promoting rhizobacteria(PGPR). This study aimed to examine the biochemical mechanism of the resistance to soybean cyst nematode ( Heterodera glycines Ichinohe ) in soybean induced by PGPR [ Sneb207 ( Bacillus megaterium) and Sneb482 (Bacillus megaterium) ]. Seed bacterization with Sneb207 and Sneb482 were utilized in the experiment. The soybean plants were inoculated with eggs of soybean cyst nematode after soybean trefoil stage and the samples of roots were obtained 6,12,18,24 and 30 days later. Activities of plant resistance correlated enzymes including defense enzymes, namely phenylalanine ammonia-lyase (PAL), polyphenoloxidase (PPO), peroxidase (POD) and chitinase were measured, the content of total phenolics was also determined. The results showed that both the activities of PAL, PPO, POD,chitinase and the contents of total phenolics could be increased significantly by seed coating with fermentation liquid of plant growth promoting rhizobacteria (Sneb207, Sneb482) in the soybean roots than those in control. Sneb207 showed greater potential in induced resistance against soybean cyst nematode in the soybean plants than Sneb482.关键词
根际促生菌/诱导抗性/胞囊线虫/防御酶/几丁质/总酚Key words
PGPR/ Induced resistance/ Heterodera glycines/ Defense enzymes/ Chitinase/ Total phenolics分类
农业科技引用本文复制引用
段玉玺,张禹,朱晓峰,刘大伟,李颂,陈立杰,王媛媛..根际促生菌诱导大豆抗大豆胞囊线虫的生化机理[J].大豆科学,2011,30(3):442-446,5.基金项目
国家现代农业产业技术体系资助项目 （）
农业部公益性行业科技专项资助项目(20090340-03). （20090340-03）
