大豆科学2011，Vol.30Issue(3)：362-368,7.
农杆菌介导的大豆子叶节遗传转化技术流程及操作要点
Technique flow and Key Operation Points of Agrobacterium-mediated Genetic Transformation of Soybean Cotyledonary Node
摘要
Abstract
The low soybean genetic transformation frequency was one of the difficult problems that needed be resolved in soybean transgenie breeding. In this study, the soybean cotyledonary node was used as explants, and the technique flow and key operation points of Agrobacterium-mediated transformation method, including Agrobacterium preparation, explant and aseptic seedling preparation, infection and co-cultivation, adventitious bud induction, shoot elongation, regeneration of transgenie plantlets, plant habituation, obtaining of antibiotic resistant detection of transgenic plants, was systematically investigated.Therefore, a stable and efficient genetic transformation system using soybean cotyledonary nodes as explants was established.Using this transformation system, A1 gene isolated from Glycine soja was transformed into four soybean cultivars, including Suinong28, Hefeng55, Hefeng50 and Dongnong50. And several important factors affecting the transformation efficiency were also discussed in the explant.关键词
大豆/遗传转化/农杆菌介导/子叶节/技术流程Key words
Soybean/ Genetic transformation/ Agrobacterium-mediated/ Cotyledonary node/ Technique flow分类
农业科技引用本文复制引用
赵晓雯,吴芳芳,狄少康,柏锡,纪巍,李勇,才华,朱延明..农杆菌介导的大豆子叶节遗传转化技术流程及操作要点[J].大豆科学,2011,30(3):362-368,7.基金项目
国家高技术研究发展计划("863"计划)资助项目(2008AA10Z153) （"863"计划）
转基因生物新品种培育科技重大专项资助项目(2008ZX08004-002). （2008ZX08004-002）
