分析测试学报2011，Vol.30Issue(6)：635-639,5.DOI:10.3969/j.issn.1004-4957.2001.06.008
多壁碳纳米管净化/超高效液相色谱串联质谱同时测定动物组织中四环素与喹诺酮多残留
Simultaneous Determination of Tetracyclines and Quinolones Residues in Animal Tissue by Ultra Performance Liquid Chromatography- Tandem Mass Spectrometry and Multiwalled Carbon Nanotubes Cleaning
摘要
Abstract
An analytical method was developed and validated for the simultaneous determination of 3 tetracyclines and 2 quinolones in pork and chicken by ultra performance liquid chromatography -tandem mass spectrometry ( UPLC - MS/MS) by positive ion mode under multiple reaction monitoring (MRM). The sample were extracted by ultrasonic extraction in Mcllvaine -Na2EDTA buffer (pH 4. 0). The extract was cleaned up on a solid phase extraction (SPE) cartridge packed with multiwalled carbon nanotubes (MWCNTs) and using formic acid - acetonitrile (5: 95, by volume) mixture as eluting solvent. The drugs residues were analyzed by UPLC - MS/MS. The analysis time was only 10 min. Good linearities were obtained in the range of 5 - 500 pg/L. The limit of detection (LOD) was 15 μg/L for chlortetracycline(CTC) and 5μg/L for the other four drugs. The recoveries at spiked levels of 50, 100, 200 μg/L ranged from 76％ to 90％ for 5 drugs with relative standard deviations(RSDs) less than 9％. With good stability and high sensitivity, the method could be used for the rapid determinaion of these drug residues in pork and chicken sample.关键词
多壁碳纳米管/超高效液相色谱-串联质谱/四环素/喹诺酮/猪肉/鸡肉Key words
multiwalled carbon nanotubes(MWCNTs)/ ultra performance liquid chromatography -tandem mass spectrometry ( UPLC - MS/MS)/ tetracyclines/ quinolones/ pork/ chicken分类
化学化工引用本文复制引用
赵海香,孙艳红,丁明玉,陈丽梅,邓维,赵孟彬..多壁碳纳米管净化/超高效液相色谱串联质谱同时测定动物组织中四环素与喹诺酮多残留[J].分析测试学报,2011,30(6):635-639,5.基金项目
北京市海淀区科委企业博士后科研专项资助项目(k2008198) （k2008198）
