分析测试学报2011，Vol.30Issue(6)：701-704,4.DOI:10.3969/j.issn.1004-4957.2001.06.022
高效液相色谱法测定化妆品中10种限用物质
Determination of 10 Kinds of Restricted Substances in Cosmetics by High Performance Liquid Chromatography with Photodiode Array Detector
摘要
Abstract
A high performance liquid chromatography (HPLC) coupled with photodiode array detector (PDAD) method was developed for the determination of content of hydroquinone, phenol, salicylic acid, 2-phenoxyethanol, methyl 4-hydroxybenzoate, ethyl 4-hydroxybenzoate, propyl 4-hydroxybenzoate, butyl 4-hydroxybenzoate, dichlorophcn and triclosan in cosmetics sample. The restricted substances were extracted with acetonitrile in sonic bathing for 10 min. The chromatographic separation was completed with a column of Lichrosorp RP-18 (250 mm ×4. 6 mm, 5 μm) using methanol acetonitrile - ammonium acetate ( pH 4. 0) as mobile phase. The quantification was earriod out by the external standard method. The calibration curves of 10 restricted substances were linear in the range of 0. 01 -0. 1 g/L. The recoveries at three spiked levels of 50, 100, 200 mg/kg ranged from 80％ to 113％. The relative standard deviations(RSD, n =7) ranged from 2. 2％ to 10. 0％ and the quantitation limits were in the range of 10 - 50 mg/kg. The method was rapid, easy, accurate and sensitive, and could meet the detection requirements for domestic and foreign limited substances in cosmetics.关键词
高效液相色谱/二极管阵列检测器/化妆品/氢醌/苯酚/三氯生/限用物质/检测Key words
HPLC/ photodiode array detector/ cosmetics/ hydroquinone/ phenol/ triclosan/ restricted substances/ determination分类
化学化工引用本文复制引用
赵孔祥,苏毅,陈旭艳,葛宝坤..高效液相色谱法测定化妆品中10种限用物质[J].分析测试学报,2011,30(6):701-704,4.基金项目
天津出入境检验检疫局科研资助项目(TK005-2007) （TK005-2007）
