草业学报2011，Vol.20Issue(3)：192-197,6.
一种有效检测水稻叶鞘低丰度蛋白的方法
An approach to detect the low-abundant proteins in rice leaf sheath
摘要
Abstract
Enrichment of low-abundant proteins and their detection still remain great challenges in proteomics research. The existence of high-abundant proteins, e. g. ribulose-1, 5-bisphosphate carboxylase/oxygenase (rubisco) in plants, leads to this dilemma. The high-abundant proteins engage a large proportion of the wholecell proteins and thus make the low-abundant proteins poorly detectable by 2-DE. In this report, we used a protocol for the preparation of whole-cell proteins through polyethylene glycol (PEG) precipitation, to develop an approach of identifying low-abundant proteins in rice leaf sheathes. In a comparison of the 2-DE analyses of protein samples prepared using the Mg/NP-40 method without PEG fractionation, with the 15 ％ and 20 ％ PEG fractionation protocol, a relatively high reproducibility was achieved using the 20％ PEG fractionation protocol in terms of protein species and low-abundant proteins.关键词
叶鞘/低丰度蛋白/PEG/双向凝胶电泳Key words
rice leaf sheath/ low-abundant proteins/ PEG/ 2-DE分类
农业科技引用本文复制引用
王莹,崔为同,杨明峰,沈世华..一种有效检测水稻叶鞘低丰度蛋白的方法[J].草业学报,2011,20(3):192-197,6.基金项目
国家高技术研究发展计划项目(No.2007AA10Z109)资助. （No.2007AA10Z109）
