中国实验动物学报2011，Vol.19Issue(3)：211-215,5.DOI:10.3969/j.issn.1005-4847.2011.03.007
甲型H1N1流感病毒在季流病毒、禽流感病毒共感染时变异可能性的验证
No mutation of influenza A (H1N1) virus at co-infection with seasonal influenza and avian influenza viruses in miniature pigs
摘要
Abstract
Objective To co-infect miniature pigs with influenza A virus A/California/7/2009 with A/Brisbane/ 10/07 and A/Shenzhen/406H/06 virus, respectively, and to verify whether the co-infection of A/California/7/2009 ( CA7 ) and A/Brisbane/10/07 (H3N2) or A/California/7/2009 and A/Shenzhen/406H/06 (H5N1) influenza viruses will induce mutation in influenza virus. Methods To co-infect the 5-6 month old miniature pigs with A/California/7/2009 (CA7) and A/Brisbane/10/07 (H3N2) or A/California/7/2009 and A/Shenzhen/406H/06 (H5N1) viruses by intranasal instillation after 0. 1 mL/kg compound ketamine anesthesia. After 5 days,the animals were euthanized and the lung tissue samples were taken for viral sequencing. Results In the A/California/7/2009 (CA7) and A/Brisbane/10/07 (H3N2) co-infected pigs,A/California/7 / 2009 virus PB1 993 gene showed G→A and PA Gene 1659 G→A mutations,with no amino acid variation. In the A/Califoruia/7/2009 and A/Shenzhen/406H/06 (H5N1) co-infected pigs,the A/California/7/2009 virus PB2 gene 1711 showed T→ C mutation, with no amino acid variation. Conclusions Our findings demonstrate that no virus recombination and mutation occur after A/California/7/2009 (CA7) and A/Brisbane/10/07 (H3N2) ,A/California/7/2009 and A/Shenzhen/406H/06 (H5N1) virus co-infection in the miniature pigs.关键词
A/California/7/2009/A/Brisbane/10/07/A/Shenzhen/406H/06/小型猪/变异Key words
Influenza A/ A/California/7/2009/ A/California/4/2009/ Co-infection/ Miniature pigs/ Mutation分类
生物科学引用本文复制引用
鲍琳琳,许黎黎,孙惠惠,占玲俊,邓巍,朱华,高虹,陈鸿霖,秦川..甲型H1N1流感病毒在季流病毒、禽流感病毒共感染时变异可能性的验证[J].中国实验动物学报,2011,19(3):211-215,5.基金项目
科技重大专项-艾滋病和病毒性肝炎等重大传染病防治(2009ZX10004-402,2009ZX10004-016) （2009ZX10004-402,2009ZX10004-016）
卫生公益性行业科研专项项目(200802036) （200802036）
中央级公益性科研院所基本科研业务费(DWS200810). （DWS200810）
