中国实验动物学报2011，Vol.19Issue(3)：224-227,4.DOI:10.3969/j.issn.1005-4847.2011.03.010
河南地方野生小鼠与近交系小鼠的扩增片段长度多态性分析
AFLP Analysis of wild mice from Henan and inbred mouse strains
摘要
Abstract
Objective To determine the genetic variation and genetic relationship between the wild mouse captured from Lankao county, Henan province, and four common strains of laboratory mice ( B6, BALB/c, DDK, PWK) ,and to construct a wild-origin inbred line mouse. Methods Totally 25 pairs primers were used to identify DNA polymorphisms that distinguish the five strains of mice by amplified fragment length polymorphism (AFLP). Results AFLP primer combinations detected a total of 2035 amplification bands. There were 507 polymorphic bands, 24. 91％ of total bands. The genetic distance index between LK mice and PWK, DDK, BALB/c, C57BL/6J mice was 0. 01696,0. 02790,0. 02729, and 0. 02759,respectively. Conclusions Th results of this study show that LK mouse and PWK mouse have the closest genetic relationship and the genetic distance index between them is the least. It also demonstrates the apparent advantages of AFLP technique in species identification, genetic background analysis and prediction of effects such as hybrid parents.关键词
野生小鼠/近交系/AFLP/遗传多态性Key words
Genetic polymorphism/ Inbred strain/ AFLP/ Wild mouse分类
生物科学引用本文复制引用
张晶,陈修栋,王善强,周鑫,郑振宇..河南地方野生小鼠与近交系小鼠的扩增片段长度多态性分析[J].中国实验动物学报,2011,19(3):224-227,4.基金项目
河南省教育厅高校杰出科研人才创新工程项目(006KYCX015). （006KYCX015）
