中国实验动物学报2011，Vol.19Issue(3)：237-241,后插6-后插7,7.DOI:10.3969/j.issn.1005-4847.2011.03.013
豚鼠动脉粥样硬化模型形成机制:LDL-C代谢异常
Mechanism of early atherosclerosis in guinea pigs: abnormal metabolism of LDL-C
摘要
Abstract
Objective To establish a model of early atherosclerosis in guinea pig and explore its pathogenetic mechanisms, compared with the modeling in rats, and to clarify the characteristics and advantages of this guinea pig model of atherosclerosis.Methods The differences of early atherosclerosis induced by high lipid diets in guinea pig and rat were investigated.Aortic intima-media thickness, intimal inflammatory cells infiltration and plaque development were observed by pathology with HE staining.Serum lipid levels were measured by enzymatic methods.serum Ox-LDL levels were detected by enzyme-linked immunosorbent assay (ELISA).The relative expressions of LDL-R mRNA in the liver were detected by realtime PCR.The protein expression of CD36 in the atherosclerotic aortic wall was evaluated by immunohistochemistry.Results Compared with the control, the arterial intima was significantly thickened, the infiltration and aggregation of monocytes and macrophages significantly increased, and plaques consisting of foam cells accumulation developed in guinea pigs of the model groups.But such changes were not observed in the rats.The mechanism analysis showed that in comparison with the control group, serum Ox-LDL concentration increased, hepatic LDL-R mRNA expression significantly decreased and CD36 expression in the aorta was markedly enhanced in guinea pigs of the model groups.Conclusion Our findings indicate that different from that in the rats, typical atherosclerosis can be developed in guinea pigs with high lipid diet for 10 weeks.The mechanism is mainly due to the involvement of abnormal metabolism of LDL-C.关键词
豚鼠/动脉粥样硬化/Ox-LDL/LDL-R/CD36Key words
Guinea pig/ Rat/ High lipid diet/ Early atherosclerosis/ LDL-R/ LDL-C/ Ox-LDL/ CD36/ Mechanism分类
生物科学引用本文复制引用
杨润梅,陈慧敏,高南南,宋鑫,李金莲,蔡大勇..豚鼠动脉粥样硬化模型形成机制:LDL-C代谢异常[J].中国实验动物学报,2011,19(3):237-241,后插6-后插7,7.基金项目
科技部"重大新药创制"科技重大专项-综合性新药研究开发技术大平台-创新药物研究开发技术平台建设(2009ZX09301-003). （2009ZX09301-003）
