中国临床药理学与治疗学2011，Vol.16Issue(5)：501-505,5.
药用注射辅料聚山梨酯80诱发类过敏反应的细胞研究
Cell research on anaphylactoid reaction induced by pharmaceutical excipients Polysorbate 80
摘要
Abstract
AIM: To observe the effects of Polysorbate 80 on basophilic cell leukemia RBL2H3 cell degranulation and discuss the possible mechanism of anaphylactoid reaction. METHODS: RBL-2H3 cells were incubated with Polysorbate 80 for 60 min, and then the morphological changes of cells degranulation were observed by transmission electron microscopy, cell degranulation rate was detected by Alcian blue dye assay, Annexin V binding assay and β-hexosaminidase assay. RESULTS: Different concentrations of Polysorbate 80 can induce the typical morphological changes in RBL-2H3 cell with degranulation. Compared with the control group,the rates of degranulation and histamine release in Polysorbate 80 treated cells were significantly increased and dose-dependent. CONCLUSION:RBL-2H3 cell degranulation, histamine can be induced by single dose Polysorbate 80, and then induced anaphylactoid reaction, which may be one of the possible mechanisms of serious adverse induced by Polysorbate 80 injection for the first administration in clinic.关键词
药用辅料/聚山梨酯80/RBL-2H3细胞/脱颗粒/类过敏反应Key words
Pharmaceutical excipients/ Polysorbate 80/ RBL-2H3 cell/ Degranulation/ Anaphylactoid reaction分类
医药卫生引用本文复制引用
李钦,赵吟,郑晓亮,张信岳..药用注射辅料聚山梨酯80诱发类过敏反应的细胞研究[J].中国临床药理学与治疗学,2011,16(5):501-505,5.基金项目
浙江省中医局青年基金(2008YA013) （2008YA013）
