中国临床药理学与治疗学2011，Vol.16Issue(5)：549-552,4.
异硫氰酸苯酯柱前衍生化RP-HPLC法测定人血浆中10种氨基酸的浓度
Simultaneous determination of 10 amino acids in serum by reversed phase high performance liquid chromatography with pre-column phenylisothiocyanate derivatization
杨智 1阳利龙 1祝文兵 1曹靖 1何周康1
作者信息
- 1. 湖南省儿童医院药剂科,长沙410007,湖南
- 折叠
摘要
Abstract
AIM: To establish a reversedphase high performance liquid chromatographic method for simultaneous determination of 10 amino acids in serum. METHODS: The sample was mixed with norleucine as the internal standard, then derivatized with phenylisothiocyanate (PITC)and analyzed on a Venusil-AA column at 40 ℃, using gradient elution with detection at 254 nm. RESULTS: The correlation coefficients between the ratios of peak area of amino acid to that of the internal standard and the amino acid concentrations were all above 0.99 in the linear range 15.6-250 μmol/L. The recoveries of amino acids added were 96.3％ - 103.4％, with-in and between day precision were less than 4.8 ％.The method was applied to analyze free amino acids in infants plasma and good results were obtained. CONCLUSION: The method is simple,stable, the separation of amino acids is satisfactory. It can be used for determination of amino acids in human plasma.关键词
高效液相色谱法/柱前衍生化/异硫氰酸苯酯/正亮氨酸/氨基酸Key words
HPLC/ Precolumn derivatization/ Phenylisothiocyanate/ Norleucine/ Amino acid分类
医药卫生引用本文复制引用
杨智,阳利龙,祝文兵,曹靖,何周康..异硫氰酸苯酯柱前衍生化RP-HPLC法测定人血浆中10种氨基酸的浓度[J].中国临床药理学与治疗学,2011,16(5):549-552,4.
