中国临床药理学与治疗学2011，Vol.16Issue(5)：553-558,6.
CTLA-4siRNA对慢性乙型肝炎患者外周血Th1/Th2细胞因子的影响
Effect of silencing CTLA4 with siRNA on the Th1/Th2 cytokines in peripheral blood of chronic hepatitis B patient
余永胜 1吴昊 1汤正好 1臧国庆1
作者信息
- 1. 上海交通大学附属第六人民医院感染病科,上海200233
- 折叠
摘要
Abstract
AIM: To explore the inhibition of expression for cytotoxic T-lymphocyte antigen 4 (CTLA-4) in peripheral blood lymphocytes in patients with CHB by chemically synthesized small interfering RNA (siRNA) and the secretion of IFN-γ, IL-2, IL-4, then to analyze the T cellular immune activation by the interference of CTLA-4 gene. METHODS: CTLA-4 of peripheral blood lymphocytes in patients with CHB was detected to observe the correlation with the HBV-DNA; according to human CTAL-4 gene sequence, CTAL-4 siRNA and the negative control (siRNA-co) were designed, synthesized,and then they were transfected into peripheral blood lymphocytes separated from CHB patients by electroporation. The expression of CTLA-4 mRNA was detected by real-time Q-PCR, the CTAL-4 protein was measured by Western Blot and the secretion of IFN-γ, IL-2 and IL-4 were assayed by enzyme-linked immunosorbent assay (ELISA). RESULTS: CTLA-4 of peripheral blood lymphocytes in patients with CHB was correlated to HBV-DNA (P＜0.05); after CTLA-4 were transfected into peripheral blood lymphocytes separated from CHB patients, the expression of CTLA-4 mRNA, CTAL-4 protein were suppressed and the production of IFN-γ,IL-2 were enhanced, compared with the negative control group (P＜0.01), while IL-4 concentrations did not show any significant differences (P ＞0.05). CONCLUSION: CTLA-4 of peripheral blood lymphocytes in patients with CHB might contribute to the persistent infection of HBV.Inhibiting the expression of CTLA-4 mRNA in peripheral blood lymphocytes in patients with CHB could up-regulate the secretion of Th1-type cytokines IFN-γ, IL-2 except Th2-type cytokines IL-4. It suggested that inhibiting CTAL-4 is helpful to activating T cellular immunity of patients with CHB.关键词
慢性乙型肝炎/细胞毒性T淋巴细胞相关抗原4/siRNA/Th1/Th2Key words
CHB/ CTLA-4/ siRNA/ Thl/Th2分类
医药卫生引用本文复制引用
余永胜,吴昊,汤正好,臧国庆..CTLA-4siRNA对慢性乙型肝炎患者外周血Th1/Th2细胞因子的影响[J].中国临床药理学与治疗学,2011,16(5):553-558,6.
