中国肿瘤生物治疗杂志2011，Vol.18Issue(3)：270-274,5.DOI:10.3872/j.issn.1007-385X.2011.03.06
雷公藤内酯醇下调P53基因甲基化抑制肝癌SMMC-7721细胞的增殖
Triptolide down-regulates P53 gene methylation and inhibits proliferation of hepatocarcinoma SMMC-7721 cells
摘要
Abstract
Objective: To study the effect of triptolide (TP) on the proliferation of hepatocarcinoma SMMC-7721 cells and its effect on demethylation of P53 gene. Methods: The effect of TP on proliferation of SMMC-7721 ceils was measured by MTT method, the effect of TP on P53 gene methylation in SMMC-7721 cells was analyzed by methylation specific PCR, the expressions of methyltransferase DNMT1, DNMT3a and DNMT3b mRNA in SMMC-7721 cells were measured by RT-PCR, and the protein expression of P53 in SMMC-7721 cells was detected by Western blotting assay. Results: TP inhibited the proliferation of SMMC-7721 cells in a dose-dependent manner ( P ＜ 0.05 ), with the inhibitory rate being (73.5 ± 3.02) ％ at 40 ng/ml TP, and IC50 of TP was 20 ng/ml. TP significantly inhibited DNMT1, DNMT3a, and DNMT3b mRNA expressions in SMMC-7721 cells (P ＜ 0.05, P ＜0.01 ) , and dose-dependently reversed the hypermethylation of P53 gene and enhanced P53 protein expression in SMMC-7721 cells. Conclusion: TP can inhibit the proliferation of SMMC-7721 cells through the inhibiting methyltransferase expression, which augment the demethylation of P53 gene and results in the increased expression of P53 protein.关键词
雷公藤内酯醇/P53基因/甲基化/SMMC-7721细胞/甲基转移酶Key words
triptolide/ P53 gene/ methylation/ SMMC-7721 cell/ methyltransferase分类
医药卫生引用本文复制引用
吴瑛,刘芳,郭文渊,肖亮,阳揭宇,谢江平,丁国善..雷公藤内酯醇下调P53基因甲基化抑制肝癌SMMC-7721细胞的增殖[J].中国肿瘤生物治疗杂志,2011,18(3):270-274,5.基金项目
国家自然科学基金资助项目(No.81072444). （No.81072444）
