中国肿瘤生物治疗杂志2011，Vol.18Issue(3)：301-305,5.DOI:10.3872/j.issn.1007-385X.2011.03.012
人外周血γδT细胞CD107a的表达变化与细胞毒活性的关系
Relationship of CD107a expression with cytotoxic activity of human peripheral γδT cells
摘要
Abstract
Objective: To investigate changes of CD107a expression in γδT cells during cultivation and the relationship of CD107a expression with cytotoxicity of γδT cells. Methods: γδT cells were generated in vitro by stimulating PBMCs with IL-2 and isopentenyl pyrophosphate (IPP). Phenotype analysis of γδT cells was performed on the 7, 10 and 14 day by flow cytometry. Meanwhile, CD107a, perforin and granzyme B expressions were detected in γδT cells by flow cytometry. The cytotoxicity of γδT cells on pancreatic carcinoma SW-1990 cells was determined by CCK-8 kit. Spearman correlation analysis was performed by SPSS13.0 software. Results: γδTCR expression in γδT cells was (60.31 ± 3.84)％,(66.45 ±4.25)％ and (70.99 ±4.66)％ on 7, 10 and 14 day, respectively. The expression of CD107a, perforin and granzyme B reached the peak on7 ～10 d (7 d vs0 d: [80.66 ±4.42]％, [70.11 ±3.34]％, [94.26 ±4.25]％ vs [ 69.02 ± 5.04 ] ％, [ 62.31 ± 4.66 ] ％, [ 53.62 ± 3.69 ] ％, P ＜ 0.05 ), and then gradually decreased. The cytotoxicity rates of 7 day and 10 day γδT cells against SW - 1990 cells were significantly higher than those of 14 day γδT cells ( [ 58.86 ±5.12] ％, [61.53 ±4.69] ％ vs [40.31 ±4.83 ] ％, P ＜0.05 ). CD107a expression in γδT cells was significantly correlated with perforin, granzyme B expressions and cytotoxicity on SW-1990 cells (P ＜0.01 ). Conclusion: The expression of CD107a on human peripheral γδT cells is positively correlated with its anti-tumor effect and may serve as a marker for the cytotoxic activity of γδT cells.关键词
γδT细胞/CD107a/胰腺肿瘤/细胞毒作用/穿孔素/颗粒酶BKey words
γδT cell/ CD107a/ pancreatic carcinoma/ cytotoxicity/ perforin/ granzyme B分类
医药卫生引用本文复制引用
周忠海,陈复兴,吕小婷,张娟,孙蕾清,张磊,黄菲..人外周血γδT细胞CD107a的表达变化与细胞毒活性的关系[J].中国肿瘤生物治疗杂志,2011,18(3):301-305,5.基金项目
南京军区医学科技创新基金资助项目(No.08MA040). （No.08MA040）
