基础医学与临床2011，Vol.31Issue(8)：869-874,6.
红霉素抑制U937细胞TREM-1受体的表达及其抗炎价值
The anti-inflammatory valument and inhibition effects of erythromycin on TREM-1 in U937 cell
摘要
Abstract
Objective To confirm the effect of erythromycin on TREM-1, and to investigate the mechanism.Methods U937 cells were cultured and divided into four groups: control group, LPS group, LPS + TREM-1/FC fusion protein group and LPS + erythromycin group.The cytokines, TNF-α, IL-6, IL-1 β and sTREM-1, were measured by ELISA.The expression of membrane receptor and gene of TREM-1 were measured by flow cytometry and RT-PCR respectively.Results The top point of membrane expression of TREM-1 was (0.65 ±0.05)and (0.72 ±0.04)in TREM-1/FC fusion protein group and erythromycin group respectively, which was lower than that in LPS group(0.98 ±0.12)(P ＜ 0.01 ).They failed to inhibit the expression of the gene.Under LPS stimulation the secretion of these cytokines was inhibited obviously.The depressant effect of TREM-1/FC fusion protein was stronger than that of erythromycin,on sTREM-1 (P＜0.01) and IL-6 (P＜0.05), but the result was contrasted on TNF-α, IL-1β(P＜0.05).Conclusion Erythromycin may inhibit the expression of TREM-1, which would depress the inflammatory reaction, and has the analogous clinical anti-inflammatory effect as TREM-1/FC fusion protein.关键词
TREM-1/红霉素/TREM-l/FC融合蛋白/炎性反应Key words
TREM-1/ erythromycin/ TREM-1/FC fusion protein/ inflammation reaction分类
医药卫生引用本文复制引用
童瑾,王导新..红霉素抑制U937细胞TREM-1受体的表达及其抗炎价值[J].基础医学与临床,2011,31(8):869-874,6.基金项目
国家自然科学基金(30971303) （30971303）
