基础医学与临床2011，Vol.31Issue(8)：879-883,5.
抑郁模型大鼠杏仁核神经元凋亡增加
Increased apoptosis in amygdala neurons of depression model rats
摘要
Abstract
Objective To observe the expression of cytochrome C and apoptosis-related gene BAX in amygdala of depression model rats. Methods Male Wistar rats were randomly divided into control group and model group.Chronic forced swimming stress was carded out to set up the depression model. TUNEL-staining and double-labeled flow cytometry (FCM) were employed for the detection and quantification of the apoptotic cells in the amygdala.The expression of cytochrome C and gene BAX were detected using Western blot and RT-PCR. Results The consumption of sucrose, preference of sucrose, erect quantity and escape latency of model rat were signficantly decreased as compared with that of normal group ( P ＜ 0. 01, P ＜ 0. 05 ). The percentage of TUNEL-positive cell, the apoptotic cell rate and the expression of cytochrome C and gene BAX in model group were higher than those from control group respectively ( P ＜ 0. 01 ). Conclusion Apoptosis is increased in amygdala neurons of depression model rats, and BAX is up-regulated and promotes the releasing of cytochrome C.关键词
慢性强迫游泳应激/杏仁核/凋亡/细胞色素C/BAXKey words
chronic forced swimming stress/ amygdala/ apoptosis/ cytochrome C/ BAX分类
医药卫生引用本文复制引用
田艳霞,王海涛,李冉,阚泉,高俊玲..抑郁模型大鼠杏仁核神经元凋亡增加[J].基础医学与临床,2011,31(8):879-883,5.基金项目
河北省自然科学基金资助项目(C2009001247) （C2009001247）
