基础医学与临床2011，Vol.31Issue(8)：889-893,5.
阿米卡星致毒豚鼠耳蜗中p-JNK表达增强
Enhanced expression of phosphorylated JNK of cochlea ototoxicity by amikacin-induced in guinea pig
摘要
Abstract
Objective To investigate the effect of amikacin (AMK) on the expression of phosphorylated JNK (p-JNK) in hair cells of guinea pig cochlea and the mechanism of AMK ototoxicity. Methods Guinea pigs were divided into control group, AMK 3, 7 and 11 days group. Animals from AMK group were daily injected intramuscular with AMK(400 mg/kg), while control animals received an equivalent volume of saline. Auditory brainstem response (ABR) test and tetramethylrhodamine isothiocyanate ( TRITC)-labeled phalloidine staining were used to observe the auditory function and hair cells morphology. immunohistochemistry and imaging analysis technique and Western blot were used to detect the expression of p-JNK in the cochlea. Results The hair cells missing speed up in the basal turn of cochlea and developed to the second turn. ( 1 ) The average absorbance values of p-JNK stained in cochlear outer hair cells, were 169. 40 ± 1.18, 153.87 ± 2. 05 and 145.23 ± 2. 98, in AMK 3, 7 and 11 days group. The expression of p-JNK was greater remarkably in AMK groups. (2) ABR threshold shifts ( dB SPL) in AMK 7 and 11 d group, at4, 12 and 24 kHz stimulation frequencies, were 6. 25 ± 1.96, 10. 58 ±2. 90, 16. 13 ±3.80 and 49. 19 ± 7. 48, 58.42 ± 7. 56, 63.54 ± 8.72. And the ABR thresholds shifts increase significantly than the control group (P ＜0. 01 ). Conclusion JNK signal pathway might participate in AMK-induced ototoxicity.关键词
C-JUN氨基末端激酶/阿米卡星/豚鼠/耳蜗Key words
C-JUN N-terminal kinase/ Amikacin/ Guinea pig/ Cochlea分类
医药卫生引用本文复制引用
刘双月,王爱梅,许涛..阿米卡星致毒豚鼠耳蜗中p-JNK表达增强[J].基础医学与临床,2011,31(8):889-893,5.基金项目
辽宁省教育厅科学研究计划(L2010271) （L2010271）
