环境与职业医学2011，Vol.28Issue(6)：338-341,4.
144例HBsAg携带者HBV基因型分布及PreS区位点的突变
Distribution and PreS Region Mutation of Hepatitis B Virus Genotypes in 144 HBsAg Carriers
摘要
Abstract
[ Objective ] To survey distribution and mutation of hepatitis B virus ( HBV ) genotype in HBsAg carriers and to provide evidence for prevention and treatment of hepatitis B. [ Methods ] Basic information of selected subjects were collected by a structured questionnaire. HBV genotypes of the subject samples were determined by a multiplex polymerase chain reaction( PCR ) assay. PCR amplification and DNA sequencing of the PreS region were performed. [ Results ] Of 144 HBsAg carriers, 8.33％ had more than one symptom related to hepatitis in a month before the survey; 17.36％ of subjects ordered hepatic function tests and 6.94％ did type B ultrasonic examination to liver section in last three years; 20.14％ of subjects discovered positive HBsAg in hospital and 79.86％ by the current survey; 61.81％ of subjects were infected by HBV genotype B and 38.19％ by HBV genotype C. In terms of proportion of HBV genotype B and C, 31.25％ and 68.75％ were found in the local resident group respectively, while 65.62％ and 34.38％ in the migrant group. Byx2 test, the distribution of HBV genotype in the two groups was significantly different( P＜0.05 ). In PreS region detection, 6.25％ of subjects with genotype C in the local resident group were identified as PreS1, 16.67％ and 6.82％ of genotype B and C in the migrant group were identified as PreS1 respectively while 2.38％ and 4.55％ were PreS2 respectively in the same group. [ Conclusion ] The distribution of HBV genotype and PreS region mutation in selected groups are delineated. Some subjects also show active HBV reproduction. It suggests a community-based surveillance system covering HBsAg carriers in order to identify and treat hepatitis in very early stage and improve effectiveness of HBV infection prevention and control.关键词
乙肝病毒/基因型/突变Key words
hepatitis B viruse/ genotype/ mutation分类
医药卫生引用本文复制引用
王琳,张蓉,金玉琴,曹英川,殷建华,马旭华..144例HBsAg携带者HBV基因型分布及PreS区位点的突变[J].环境与职业医学,2011,28(6):338-341,4.基金项目
上海市卫生局项目基金资助(编号:2008206) （编号:2008206）
