环境与职业医学2011，Vol.28Issue(7)：387-392,6.
正己烷外周神经系统损伤早期效应标志物研究
Study on Early Effect Biomarkers of Peripheral Nervous System Damage Induced by n-Hexane
摘要
Abstract
[ Objective ] To determine the valuable significance of myelin basic protein ( MBP ), neuron-specific enolase ( NSE ), nerve growth factor ( NGF ), and neurofilament ( NF ) in serum as early effect biomarkers of peripheral nervous system damage induced by n-hexane. [ Methods ] n-Hexane was administered by garage to rats sub-chronically. Peripheral nerve damage was verified by specific symptoms and pathological examination. Simultaneously MBP, NSE, NGF, and NF levels in serum of rats were detected. [ Results ] Typical symptoms of multiple peripheral neuropathy appeared in rats exposed to n-hexane, nerve damage was confirmed by pathological examination. Serum NSE and NF levels significantly increased starting from 1st or 2nd week after intoxicated in different dose groups, respectively( P＜0.05 ), the maximum raise were 122.94 ％ and 193.35 ％ respectively.Serum NGF and MBP levels changed only in very few time points. Pearson correlation coefficient between gait score and serum NSE, NF, NGF, MBP were 0.608( P＜0.05 ), 0.595( P＜0.05 ), -0.284( P＞0.05 ), and 0.179( P＞0.05 )respectively. Bench mark dose ( BMD ) and bench mark dose lower confidence limit( BMDL )calculated according to NSE and NF were about 150 mg/( kg· d )and 105 mg/( kg·d ). [ Conclusion ] Serum NSE, NF were the most sensitive indicators of peripheral nervous system damage,whose increase preceded the symptom manifested in the animals intoxicated and changed substantially, so they could be used as good biomarkers of toxic effect induced by n-hexane. NGF had poor sensitivity in this study. Significance of MBP remained to be verified if used as a biomarker of toxic effect induced by n-hexane.关键词
正已烷/效应标志物/外周神经系统损伤Key words
n-hexane/ effect biomarker/ peripheral nervous system injury分类
医药卫生引用本文复制引用
赵乾魁,顾锡安,周志俊,周元陵..正己烷外周神经系统损伤早期效应标志物研究[J].环境与职业医学,2011,28(7):387-392,6.基金项目
国家"十一五科技支撑计划任务"(编号:2006BAI06B01) （编号:2006BAI06B01）
上海市公共卫生重点学科建设计划(编号 08GWZX0303,08GWD20) （编号 08GWZX0303,08GWD20）
