环境与职业医学2011，Vol.28Issue(7)：426-429,4.
职业性镍接触工人DNA链断裂的研究
Study on DNA Strand Breaks in Stainless Steel Workers Exposed to Nickel
郭庆峰 1孙建娅2
作者信息
- 1. 山西医科大学公共卫生学院劳动卫生学教研室,山西太原030001
- 2. 太原钢铁(集团)有限公司疾病预防控制中心职业病科,山西,太原,030003
- 折叠
摘要
Abstract
[Objective]To explore DNA damage of peripheral lymphocyte of wokers exposed to nickel in a stainless steel refining factory.[Methods]Occupational hazardous factors in a stainless steel factory were monitored, a total of 165 nickel exposed workers were recruited, including 60 steel refining workers, 65 slaggers and 40 millmen.In addition, 67 workers in water treatment department not exposured to nickel were selected and matched with those exposed workers by age and cigarette smoking.Comet assay was conducted for DNA damage of lymphocyte isolated from peripheral blood samples of all participating subjects.[Results]The comet assay indicated that the exposed workers had greater DNA damage reflected by tail inertia, tail length and olive tail moment( OTM )all different from control subjects( P＜0.01 ).Tail inertia of the steel refining workers( 23.33 ± 7.10 )and of the slaggers( 22.87 ± 10.67 )were obviously higher than the control( 10.53 ± 5.08 )( P＜ 0.01 ).Tail inertia of the millmen( 21.67 ± 9.32 )was also higher than the control ( P＜ 0.05 ).Tail length of the steel refining workers ( 1.20 ± 0.21 ), of the slaggers ( 1.18 ± 0.22 )and of the millmen ( 1.14 ± 0.24 ) were obviously higher than the control ( 0.91 ± 0.19 ) too.The OTM of the steel refining workers ( 0.495 ± 0.055 ), of the slaggers( 0.455 ± 0.045 )and of the millmen( 0.429 ± 0.069 )were all significantly higher than the control( P＜0.01 ).Significant differences were showed in the OTM values between non-smoking and smoking workers.Correlation analysis showed that there was a significant positive correlation between the urine concentration of nickel and the OTM( r=0.576,P＜0.001 ).[Conclusion]DNA strand break in lymphocytes should be an earlier impairment of occupational nickel-exposed population.Comet assay may be used for early monitoring of occupational nickel-exposed population.关键词
不锈钢冶炼/镍/职业接触/彗星试验/DNA损伤Key words
stainless steel refining/ nickel/ occupational exposure/ comet assay/ DNA damage分类
医药卫生引用本文复制引用
郭庆峰,孙建娅..职业性镍接触工人DNA链断裂的研究[J].环境与职业医学,2011,28(7):426-429,4.
