针刺研究2011，Vol.36Issue(3)：176-180,5.
针刺对结肠吻合术后大鼠干细胞因子-酪氨酸激酶受体系统的调整作用
Effect of Acupuncture on Gastrointestinal Stem Cell Factor/Kit System after Colocolic Anastomosis in Rats
邓晶晶 1刘晓嵘 2袁青3
作者信息
- 1. 广州市第八人民医院中西医科,广州,510060
- 2. 丰都县中医院针灸科,重庆,408200
- 3. 广州中医药大学针灸推拿学院,广州,510006
- 折叠
摘要
Abstract
Objective To observe the effect of acupuncture on colonic stem cell factor (SCF)-receptor tyrosine kinase (Kit, a transmembrane protein c-kit) system in colocolic anastomosis rats so as to explore its underlying mechanism in regulating intestinal motility after enteroenterostomy.Methods Thirty SD rats were randomized into control, model (colocolic anastomosis) and acupuncture groups(n = 10/group).Acupuncture was applied to bilateral “Zusanli” (ST 36), “Sanyinjiao” (SP 6) and “Taichong” (LR 3), once a day for 3 days.The fecal-excretion time and the propulsive rate of the small intestine were measured.The immunoactivity of c-kit immunoreaction positive products in the colon tissue 2 cm below the caecum was detected using immunohistochemistry and the expression of SCF mRNA detected using real time-polymerase chain reaction (RT-PCR) method.Results Following colocolic anastomosis, the intestinal propulsive rate in the model group was decreased considerably in comparison with the control group (P＜0.01).Compared with the model group, the first fecal-excretion time and the intestinal propulsive rate were improved significantly in the acupuncture group (P＜0.05).In comparison with the control group, the immunoactivity of c-kit and the expression of SCF mRNA in colon tissue were down-regulated obviously in the model group ( P＜0.05,P＜0.01 ), while in comparison with the model group, the expression levels of c-kit and SCF mRNA were increased in the acupuncture group significantly(P＜0.05).Conclusion Acupuncture can suppress enteroenterostomy-induced down-regulation of the expression of c-kit protein and SCF mRNA in colocolic anastomosis rats, which may contribute to its effect in regulating the intestinal motility after enteroenterostomy.关键词
结肠吻合术/针刺/小肠推进率/酪氨酸激酶蛋白表达/干细胞因子基因表达Key words
Colocolic anastomosis/ Acupuncture/ Intestinal propulsive rate/ c-kit protein/ SCF mRNA分类
医药卫生引用本文复制引用
邓晶晶,刘晓嵘,袁青..针刺对结肠吻合术后大鼠干细胞因子-酪氨酸激酶受体系统的调整作用[J].针刺研究,2011,36(3):176-180,5.
