针刺研究2011，Vol.36Issue(3)：181-186,6.
不同穴位针刺对进行性力竭游泳大鼠免疫平衡的保护作用
Effect of Acupuncture of Different Acupoints on Immune Function in Rats with Exhausted Swimming
摘要
Abstract
Objective To observe the effect of acupuncture of "Zusanli" (ST 36) on immune function in progressively exhausted swimming rats so as to reveal its mechanism underlying improvement of strenuous exercise. Methods Thirty-two SD rats were randomly allocated into control group, strenuous exercise (model) group, acupuncture-Xuehai (SP 10) group, Acupuncture-Zusanli (ST 36) group (n =8/group), The rats were forced to have a swimming in a water tank for 15-90 min in the first 8 days (once daily), then, a progressively exhausted load swimming 1 -3 times everyday from day 9 to 13. Bilateral SP 10 and ST 36 were punctured with filiform needles and stimulated with uniform reinforcing and reducing manipulation, once daily, after termination of the swimming and for 13 days. Serum interferon-gamma (IFN-γ) and interleukin-4 (IL-4) contents were assayed by using enzyme linked immunosorbent assay. The rat's body weight and the spleen weight were weighted by using an electronic balance for calculating the spleen index (spleen weight/body weight × 100％ ) after killing the rat under deep anesthesia. Results Compared with the model group, the time of swimming-induced exhaustion appearing at the first time from day 9 to day 13 in the SP 10 and ST 36 groups was apparently lengthened (P＜0.01). No significant difference was found between SP 10 and ST 36 groups in the time of swimming-induced exhaustion appearing at the first time of the forced swimming. Compared with the control group, the spleen index, serum IFN-γ contents and IFN-γ/IL-4 in the model group were decreased significantly (P＜0.01,P＜0.05). In comparison witl the model group, the serum IL-4 contents in the SP 10 and ST 36 groups were decreased markedly (P＜0.05,P＜0.01), and serum IFN-γ content and IFN-γ/IL-4 in the ST 36 group were increased significantly (P＜0.05,P＜0. 01). The IFN-γ level was significantly higher in the ST 36 group than in the SP 10 group (P＜0.05). No significant differences were found between SP 10 and ST 36 groups in the spleen index, IL-4 and IFN-γ/IL-4 (P＞0.05). Conclusion Acupuncture at "Zusanli" (ST 36) can lengthen the time of forced swimming-induced exhaustion, and upregulate serum IFN-γ content and IFN-γ/IL-4 in exhausted swimming rats, which may contribute to its effect in correcting Th I/Th 2 imbalance after strenuous exercise. The effect of acupuncture of ST 36 is superior to that of acupuncture of SP 10.关键词
针刺/力竭实验/干扰素-γ/白细胞介素-4/干扰素-γ/白细胞介素-4Key words
Acupuncture/ Strenuous exercise/ Serum IFN-γ/ IL-4/ IFN-γ/IL-4分类
医药卫生引用本文复制引用
张玮,赵广高,苏利强,郑里翔,严志宏..不同穴位针刺对进行性力竭游泳大鼠免疫平衡的保护作用[J].针刺研究,2011,36(3):181-186,6.基金项目
江西省教育厅科学技术研究项目(GJJ 09284) （GJJ 09284）
