中国当代儿科杂志2011，Vol.13Issue(7)：573-576,4.
孕鼠叶酸缺乏对幼鼠肺发育及其SP-A变化的研究
Effects of maternal deficiency of folic acid during pregnancy on pulmonary development and SP-A expression in newborn rats
摘要
Abstract
Objective This study examined the effects of maternal deficiency of folic acid during pregnancy on pulmonary development and protein A (SP-A) expression in newborn rats in order to explore the possible mechanism of lung developmental disorders.Methods Thirty-six adult Sprague-Dawley female rats were randomly assigned into two groups: control and study (n = 18).The study and the control groups were fed with fodder containing folic acid or not respectively.Two weeks later, the female rats in the two groups copulated with normal male rats.Newborn rats were sacrificed at 1,7 and 14 days after birth (8 pups at each time point).Lung sections were stained with hematoxylin and eosin for histological examination.SP-A expression of protein and mRNA were determined by immunohistochemistry and real-time quantitative RT-PCR, respectively.Results The newborn rats from the study group showed damaged lung tissue structures.The mean optical density of type Ⅱ cells with positive expression of SP-A decreased significantly from 1 to 14 days in newborn rats of the study group compared with the control newborn rats (P ＜0.05).The real-time quantitative RT-PCR showed that the expression of lung SP-A mRNA also decreased significantly from 1 to 14 days in newborn rats of the study group compared with control newborn rats ( P ＜ 0.05 ).Conclusions Maternal deficiency of folic acid during pregnancy can decrease the expression of SP-A in lung tissues of newborn rats, which might lead to the disorder of lung development maturation.关键词
叶酸/肺表面活性物质相关蛋白A/新生大鼠Key words
Folic acid/ Surfactant protein A/ Newborn rats分类
医药卫生引用本文复制引用
乔立兴,余章斌,韩树萍,顾筱琪,陈玉林,沙莉,金俊霞,晏路标,郭锡熔..孕鼠叶酸缺乏对幼鼠肺发育及其SP-A变化的研究[J].中国当代儿科杂志,2011,13(7):573-576,4.基金项目
江苏省自然科学基金资助项目(BK2008079) （BK2008079）
南京市医学科技发展基金重点资助项目(ZKX08002) （ZKX08002）
南京医科大学科技发展基金重点资助项目(08NMUZ036). （08NMUZ036）
